This is a correction to Krstevski, C., Cohen, C.D., Dona, M.S.I. and Pinto, A.R. (2020) New perspectives of the cardiac cellular landscape: mapping cellular mediators of cardiac fibrosis using single-cell transcriptomics. Biochem. Soc. Trans.48, 2483–2493. 10.1042/BST20191255
Following publication of the above article, the authors noticed a citation was missing from the revised article resulting in errors in Table 1, where the reference numbers associated with studies summarised in the table are incorrect.
| Context . | Reference . | Cells . | Mean Reads/cell . | Technology . | Notes and key findings . |
|---|---|---|---|---|---|
| Mouse myocardial infarction | (101) | 426 | 16,874 | Sort-Seq | • 3-days post-MI and sham control mice • n = 3/group. • Ckap4 proposed as a novel driver of myofibroblast differentiation. |
| Mouse myocardial infarction | (62) | 13,331 | ∼35,000 | 10X Chromium | • 3- and 7-days post-MI and sham control. • n=1/time point/group. • Identified heterogeneous fibroblast and myofibroblast subsets involved in fibrosis. |
| Mouse hypertension (Angiotensin II infusion) | (56) | 29,615 | ∼80,000 | 10X Chromium | • 2 weeks AngII-induced experimental hypertension and control mice. • n=4/sex/group. • Novel fibroblasts Fibro-Cilp and Fibro-Thbs4 implicated in AngII-induced fibrosis. • Almost all cardiac cell types contribute to ECM remodeling after AngII treatment. • Extensive cell and sex-specific gene expression patterns in the non-stressed and stressed hearts. |
| Mouse myocardial infarction | (66) | 36,874 | ∼46,000 | 10X Chromium | • 1, 3, 5, 7, 14- and 28-days post-MI and sham control. • n= ∼3/group. • Identified heterogeneous fibroblast and myofibroblast subsets involved in fibrosis. • Early differentiation of myofibroblasts are associated with cardiac rupture. |
| Healthy and heart failure human patients | (100) | 21,422 | ∼300,000 (Median reads/cell) | iCell8 | • Healthy organ donors and patients with HF. • n=14 healthy controls, 8 HF patients. • Endothelial cells and fibroblasts are key players in cellular crosstalk in progression of HF. |
| Context . | Reference . | Cells . | Mean Reads/cell . | Technology . | Notes and key findings . |
|---|---|---|---|---|---|
| Mouse myocardial infarction | (101) | 426 | 16,874 | Sort-Seq | • 3-days post-MI and sham control mice • n = 3/group. • Ckap4 proposed as a novel driver of myofibroblast differentiation. |
| Mouse myocardial infarction | (62) | 13,331 | ∼35,000 | 10X Chromium | • 3- and 7-days post-MI and sham control. • n=1/time point/group. • Identified heterogeneous fibroblast and myofibroblast subsets involved in fibrosis. |
| Mouse hypertension (Angiotensin II infusion) | (56) | 29,615 | ∼80,000 | 10X Chromium | • 2 weeks AngII-induced experimental hypertension and control mice. • n=4/sex/group. • Novel fibroblasts Fibro-Cilp and Fibro-Thbs4 implicated in AngII-induced fibrosis. • Almost all cardiac cell types contribute to ECM remodeling after AngII treatment. • Extensive cell and sex-specific gene expression patterns in the non-stressed and stressed hearts. |
| Mouse myocardial infarction | (66) | 36,874 | ∼46,000 | 10X Chromium | • 1, 3, 5, 7, 14- and 28-days post-MI and sham control. • n= ∼3/group. • Identified heterogeneous fibroblast and myofibroblast subsets involved in fibrosis. • Early differentiation of myofibroblasts are associated with cardiac rupture. |
| Healthy and heart failure human patients | (100) | 21,422 | ∼300,000 (Median reads/cell) | iCell8 | • Healthy organ donors and patients with HF. • n=14 healthy controls, 8 HF patients. • Endothelial cells and fibroblasts are key players in cellular crosstalk in progression of HF. |
The authors apologise for the error and would like to correct the record by including a citation to the following paper as no. 101:
Gladka MM, Molenaar B, de Ruiter H, van der Elst S, Tsui H, Versteeg D, et al. Single-Cell Sequencing of the Healthy and Diseased Heart Reveals Cytoskeleton-Associated Protein 4 as a New Modulator of Fibroblasts Activation. Circulation. 2018 Jul 10;138(2):166–180.
And updating the reader to the corrected table below:
