There is increasing appreciation among researchers and clinicians of the value of investigating biology and pathobiology at the level of cellular kinase (kinome) activity. Kinome analysis provides valuable opportunity to gain insights into complex biology (including disease pathology), identify biomarkers of critical phenotypes (including disease prognosis and evaluation of therapeutic efficacy), and identify targets for therapeutic intervention through kinase inhibitors. The growing interest in kinome analysis has fueled efforts to develop and optimize technologies that enable characterization of phosphorylation-mediated signaling events in a cost-effective, high-throughput manner. In this review, we highlight recent advances to the central technologies currently available for kinome profiling and offer our perspectives on the key challenges remaining to be addressed.
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February 2017
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Cover Image
The surface of the catalytic subunit of protein phosphatase PP1 (central 3D-structure) has many binding sites for regulatory proteins that are embedded in regulatory networks (coloured circles linked by lines). Please see pp. 89–99 for more information. Image provided by Mathieu Bollen.
Review Article|
February 15 2017
Technological advances for interrogating the human kinome
Akanksha Baharani;
Akanksha Baharani
1Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
2Vaccine and Infectious Disease Organization — International Vaccine Center, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
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Brett Trost;
Brett Trost
3The Centre for Applied Genomics, Genetics and Genome Biology, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada
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Anthony Kusalik;
Anthony Kusalik
4Department of Computer Science, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C9, Canada
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Scott Napper
1Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
2Vaccine and Infectious Disease Organization — International Vaccine Center, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
Correspondence: Scott Napper (scott.napper@usask.ca)
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Publisher: Portland Press Ltd
Received:
June 07 2016
Revision Received:
October 20 2016
Accepted:
October 25 2016
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society
2017
Biochem Soc Trans (2017) 45 (1): 65–77.
Article history
Received:
June 07 2016
Revision Received:
October 20 2016
Accepted:
October 25 2016
Citation
Akanksha Baharani, Brett Trost, Anthony Kusalik, Scott Napper; Technological advances for interrogating the human kinome. Biochem Soc Trans 8 February 2017; 45 (1): 65–77. doi: https://doi.org/10.1042/BST20160163
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