The β-barrel outer membrane proteins (OMPs) are integral membrane proteins that reside in the outer membrane of Gram-negative bacteria and perform a diverse range of biological functions. Synthesized in the cytoplasm, OMPs must be transported across the inner membrane and through the periplasmic space before they are assembled in the outer membrane. In Escherichia coli, Skp, SurA and DegP are the most prominent factors identified to guide OMPs across the periplasm and to play the role of quality control. Although extensive genetic and biochemical analyses have revealed many basic functions of these periplasmic proteins, the mechanism of their collaboration in assisting the folding and insertion of OMPs is much less understood. Recently, biophysical approaches have shed light on the identification of the intricate network. In the present review, we summarize recent advances in the characterization of these key factors, with a special emphasis on the multifunctional protein DegP. In addition, we present our proposed model on the periplasmic quality control in biogenesis of OMPs.
Skip Nav Destination
Article navigation
April 2015
-
Cover Image
Cover Image
- PDF Icon PDF LinkTable of Contents
Conference Article|
April 07 2015
Periplasmic quality control in biogenesis of outer membrane proteins
Zhi Xin Lyu;
Zhi Xin Lyu
*Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, and Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China
Search for other works by this author on:
Xin Sheng Zhao
Xin Sheng Zhao
1
*Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, and Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China
1To whom correspondence should be addressed (emailzhaoxs@pku.edu.cn).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
August 06 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2015 Biochemical Society
2015
Biochem Soc Trans (2015) 43 (2): 133–138.
Article history
Received:
August 06 2014
Citation
Zhi Xin Lyu, Xin Sheng Zhao; Periplasmic quality control in biogenesis of outer membrane proteins. Biochem Soc Trans 1 April 2015; 43 (2): 133–138. doi: https://doi.org/10.1042/BST20140217
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.