Glycolysis is an ancient biochemical pathway that breaks down glucose into pyruvate to produce ATP. The structural and catalytic properties of glycolytic enzymes are well-characterized. However, there is growing appreciation that these enzymes participate in numerous moonlighting functions that are unrelated to glycolysis. Recently, chemical genetics has been used to discover novel moonlighting functions in glycolytic enzymes. In the present mini-review, we introduce chemical genetics and discuss how it can be applied to the discovery of protein moonlighting. Specifically, we describe the application of chemical genetics to uncover moonlighting in two glycolytic enzymes, enolase and glyceraldehyde dehydrogenase. This led to the discovery of moonlighting roles in glucose homoeostasis, cancer progression and diabetes-related complications. Finally, we also provide a brief overview of the latest progress in unravelling the myriad moonlighting roles for these enzymes.
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December 2014
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Conference Article|
November 17 2014
Chemical genetics and its application to moonlighting in glycolytic enzymes
Da-Woon Jung;
Da-Woon Jung
1
*New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea
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Woong-Hee Kim;
Woong-Hee Kim
1
*New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea
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Darren R. Williams
Darren R. Williams
2
*New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea
2To whom correspondence should be addressed (emaildarren@gist.ac.kr).
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Publisher: Portland Press Ltd
Received:
July 22 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (6): 1756–1761.
Article history
Received:
July 22 2014
Citation
Da-Woon Jung, Woong-Hee Kim, Darren R. Williams; Chemical genetics and its application to moonlighting in glycolytic enzymes. Biochem Soc Trans 1 December 2014; 42 (6): 1756–1761. doi: https://doi.org/10.1042/BST20140201
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