The double-membraned autophagosome organelle is an integral part of autophagy, a process that recycles cellular components by non-selectively engulfing and delivering them to lysosomes where they are digested. Release of metabolites from this process is involved in cellular energy homoeostasis under basal conditions and during nutrient starvation. Selective engulfment of protein aggregates and dysfunctional organelles by autophagosomes also prevents disruption of cellular metabolism. Autophagosome formation in animals is crucially dependent on the unique conjugation of a group of ubiquitin-like proteins in the microtubule-associated proteins 1A/1B light chain 3 (LC3) family to the headgroup of phosphatidylethanolamine (PE) lipids. LC3 lipidation requires a cascade of ubiquitin-like ligase and conjugation enzymes. The present review describes recent progress and discovery of the direct interaction between the PtdIns3P effector WIPI2b and autophagy-related protein 16-like 1 (Atg16L1), a component of the LC3-conjugation complex. This interaction makes the link between endoplasmic reticulum (ER)-localized production of PtdIns3P, triggered by the autophagy regulatory network, and recruitment of the LC3-conjugation complex crucial for autophagosome formation.
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October 2014
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Conference Article|
September 18 2014
WIPI2b and Atg16L1: setting the stage for autophagosome formation
Michael I. Wilson;
Michael I. Wilson
*The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, U.K.
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Hannah C. Dooley;
Hannah C. Dooley
†London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3LY, U.K.
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Sharon A. Tooze
Sharon A. Tooze
1
†London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3LY, U.K.
1To whom correspondence should be addressed (emailsharon.tooze@cancer.org.uk).
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Publisher: Portland Press Ltd
Received:
June 18 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (5): 1327–1334.
Article history
Received:
June 18 2014
Citation
Michael I. Wilson, Hannah C. Dooley, Sharon A. Tooze; WIPI2b and Atg16L1: setting the stage for autophagosome formation. Biochem Soc Trans 1 October 2014; 42 (5): 1327–1334. doi: https://doi.org/10.1042/BST20140177
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