The high degree of protein sequence similarity in the MUPs (major urinary proteins) poses considerable challenges for their individual differentiation, analysis and quantification. In the present review, we discuss MS approaches for MUP quantification, at either the protein or the peptide level. In particular, we describe an approach to multiplexed quantification based on the design and synthesis of novel proteins (QconCATs) that are concatamers of quantification standards, providing a simple route to the generation of a set of stable-isotope-labelled peptide standards. The MUPs pose a particular challenge to QconCAT design, because of their sequence similarity and the limited number of peptides that can be used to construct the standards. Such difficulties can be overcome by careful attention to the analytical workflow.
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August 2014
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Conference Article|
August 11 2014
The complexity of protein semiochemistry in mammals
Robert J. Beynon;
Robert J. Beynon
1
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
1To whom correspondence should be addressed (emailr.beynon@liv.ac.uk).
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Stuart D. Armstrong;
Stuart D. Armstrong
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
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Guadalupe Gómez-Baena;
Guadalupe Gómez-Baena
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
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Victoria Lee;
Victoria Lee
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
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Deborah Simpson;
Deborah Simpson
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
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Jennifer Unsworth;
Jennifer Unsworth
*Protein Function Group, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
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Jane L. Hurst
Jane L. Hurst
†Mammalian Behaviour and Evolution Group, Institute of Integrative Biology, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, U.K.
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Publisher: Portland Press Ltd
Received:
May 12 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (4): 837–845.
Article history
Received:
May 12 2014
Citation
Robert J. Beynon, Stuart D. Armstrong, Guadalupe Gómez-Baena, Victoria Lee, Deborah Simpson, Jennifer Unsworth, Jane L. Hurst; The complexity of protein semiochemistry in mammals. Biochem Soc Trans 1 August 2014; 42 (4): 837–845. doi: https://doi.org/10.1042/BST20140133
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