Pre-mRNA (precursor mRNA) splicing is a key step in cellular gene expression where introns are excised and exons are ligated together to produce mature mRNA. This process is catalysed by the spliceosome, which consists of five snRNPs (small nuclear ribonucleoprotein particles) and numerous protein factors. Assembly of these snRNPs and associated proteins is a highly dynamic process, making it challenging to study the conformational rearrangements and spliceosome assembly kinetics in bulk studies. In the present review, we discuss recent studies utilizing techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis.
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Conference Article|
August 11 2014
Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome
Chandani Warnasooriya;
Chandani Warnasooriya
*Department of Medicine, Section of Virology and Single Molecule Imaging Group, MRC Clinical Centre, Imperial College London, London W12 0NN, U.K.
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David Rueda
David Rueda
1
*Department of Medicine, Section of Virology and Single Molecule Imaging Group, MRC Clinical Centre, Imperial College London, London W12 0NN, U.K.
1To whom correspondence should be addressed (emaildavid.rueda@imperial.ac.uk).
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Publisher: Portland Press Ltd
Received:
April 29 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (4): 1211–1218.
Article history
Received:
April 29 2014
Citation
Chandani Warnasooriya, David Rueda; Single-molecule fluorescence-based studies on the dynamics, assembly and catalytic mechanism of the spliceosome. Biochem Soc Trans 1 August 2014; 42 (4): 1211–1218. doi: https://doi.org/10.1042/BST20140105
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