CRISPR (clustered regularly interspaced short palindromic repeats) arrays and Cas (CRISPR-associated) proteins confer acquired resistance against mobile genetic elements in a wide range of bacteria and archaea. The phytopathogen Pectobacterium atrosepticum SCRI1043 encodes a single subtype I-F CRISPR system, which is composed of three CRISPR arrays and the cas operon encoding Cas1, Cas3 (a Cas2–Cas3 fusion), Csy1, Csy2, Csy3 and Cas6f (Csy4). The CRISPR arrays are transcribed into pre-crRNA (CRISPR RNA) and then processed by Cas6f to generate crRNAs. Furthermore, the formation of Cas protein complexes has been implicated in both the interference and acquisition stages of defence. In the present paper, we discuss the development of tightly controlled ‘programmable’ CRISPR arrays as tools to investigate CRISPR–Cas function and the effects of chromosomal targeting. Finally, we address how chromosomal targeting by CRISPR–Cas can cause large-scale genome deletions, which can ultimately influence bacterial evolution and pathogenicity.
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Conference Article|
November 20 2013
The subtype I-F CRISPR–Cas system influences pathogenicity island retention in Pectobacterium atrosepticum via crRNA generation and Csy complex formation
Corinna Richter;
Corinna Richter
*Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand
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Peter C. Fineran
Peter C. Fineran
1
*Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand
1To whom correspondence should be addressed (emailpeter.fineran@otago.ac.nz).
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Publisher: Portland Press Ltd
Received:
July 15 2013
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem Soc Trans (2013) 41 (6): 1468–1474.
Article history
Received:
July 15 2013
Citation
Corinna Richter, Peter C. Fineran; The subtype I-F CRISPR–Cas system influences pathogenicity island retention in Pectobacterium atrosepticum via crRNA generation and Csy complex formation. Biochem Soc Trans 1 December 2013; 41 (6): 1468–1474. doi: https://doi.org/10.1042/BST20130151
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