Membrane traffic pathways play an essential role in cells, providing a mechanism for organelles of the endomembrane system to communicate and exchange material between each other. A significant number of infections and diseases are associated with trafficking pathways, and as such gaining a greater understanding of their regulation is essential. Fluorescence-based imaging techniques are widely used to probe the trafficking machinery within cells, and many of these methods have the potential to be applied in a quantitative manner. In the present mini-review, we highlight several recent examples of how image intensity, kinetic measurements, co-localization and texture feature analysis have been used to study the function of one key family of membrane traffic regulators, the Rab GTPases. We give specific emphasis to the importance of the quantitative nature of these recent studies and comment on their potential applicability to a high-throughput format.
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December 2012
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Conference Article|
November 21 2012
Quantitative image analysis approaches for probing Rab GTPase localization and function in mammalian cells
Vasanth R. Singan;
Vasanth R. Singan
*School of Biology and Environmental Science & Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
†School of Medicine and Medical Science, University College Dublin, Belfield, Dublin 4, Ireland
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Kenan Handzic;
Kenan Handzic
*School of Biology and Environmental Science & Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
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Jeremy C. Simpson
Jeremy C. Simpson
1
*School of Biology and Environmental Science & Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
1To whom correspondence should be addressed (emailjeremy.simpson@ucd.ie).
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Publisher: Portland Press Ltd
Received:
July 09 2012
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2012 The Authors Journal
2012
Biochem Soc Trans (2012) 40 (6): 1389–1393.
Article history
Received:
July 09 2012
Citation
Vasanth R. Singan, Kenan Handzic, Jeremy C. Simpson; Quantitative image analysis approaches for probing Rab GTPase localization and function in mammalian cells. Biochem Soc Trans 1 December 2012; 40 (6): 1389–1393. doi: https://doi.org/10.1042/BST20120145
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