SERRS (surface-enhanced resonance Raman scattering) is a vibrational technique, whereby a relatively weak Raman scattering effect is enhanced through the use of a visible chromophore and a roughened metal surface. The direct analysis of DNA by SERRS requires the modification of a nucleic acid sequence to incorporate a chromophore, and adsorption of the modified sequence on to a roughened metal surface. Aggregated metallic nanoparticles are commonly used in the analysis of dye-labelled DNA by SERRS, allowing for detection levels that rival those gained from standard fluorescence-based techniques. In the present paper, we report on how SERRS can be exploited for the analysis of clinically relevant DNA samples. We also report on the ability of nanoparticles to aggregate as the result of a biologically significant event, as opposed to the use of an external charge-modifying agent. The self-assembly of metallic nanoparticles is shown to be a promising new technique in the move towards extremely sensitive methods of DNA analysis by SERRS.
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August 2012
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Conference Article|
July 20 2012
Nanoparticle assembly for sensitive DNA detection using SERRS
Kristy S. McKeating;
Kristy S. McKeating
1Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, U.K.
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Jennifer A. Dougan;
Jennifer A. Dougan
1Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, U.K.
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Karen Faulds
Karen Faulds
1
1Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, U.K.
1To whom correspondence should be addressed (emailKaren.faulds@strath.ac.uk).
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Publisher: Portland Press Ltd
Received:
January 12 2012
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biochem Soc Trans (2012) 40 (4): 597–602.
Article history
Received:
January 12 2012
Citation
Kristy S. McKeating, Jennifer A. Dougan, Karen Faulds; Nanoparticle assembly for sensitive DNA detection using SERRS. Biochem Soc Trans 1 August 2012; 40 (4): 597–602. doi: https://doi.org/10.1042/BST20120007
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