Membrane proteins are drug targets for a wide range of diseases. Having access to appropriate samples for further research underpins the pharmaceutical industry's strategy for developing new drugs. This is typically achieved by synthesizing a protein of interest in host cells that can be cultured on a large scale, allowing the isolation of the pure protein in quantities much higher than those found in the protein's native source. Yeast is a popular host as it is a eukaryote with similar synthetic machinery to that of the native human source cells of many proteins of interest, while also being quick, easy and cheap to grow and process. Even in these cells, the production of human membrane proteins can be plagued by low functional yields; we wish to understand why. We have identified molecular mechanisms and culture parameters underpinning high yields and have consolidated our findings to engineer improved yeast host strains. By relieving the bottlenecks to recombinant membrane protein production in yeast, we aim to contribute to the drug discovery pipeline, while providing insight into translational processes.
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June 2011
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Conference Article|
May 20 2011
Understanding the yeast host cell response to recombinant membrane protein production
Zharain Bawa;
Zharain Bawa
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Charlotte E. Bland;
Charlotte E. Bland
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Nicklas Bonander;
Nicklas Bonander
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Nagamani Bora;
Nagamani Bora
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Stephanie P. Cartwright;
Stephanie P. Cartwright
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Michelle Clare;
Michelle Clare
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Matthew T. Conner;
Matthew T. Conner
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Richard A.J. Darby;
Richard A.J. Darby
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Marvin V. Dilworth;
Marvin V. Dilworth
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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William J. Holmes;
William J. Holmes
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Mohammed Jamshad;
Mohammed Jamshad
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Sarah J. Routledge;
Sarah J. Routledge
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Stephane R. Gross;
Stephane R. Gross
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
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Roslyn M. Bill
Roslyn M. Bill
1
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, U.K.
1To whom correspondence should be addressed (email r.m.bill@aston.ac.uk)
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Publisher: Portland Press Ltd
Received:
February 10 2011
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (3): 719–723.
Article history
Received:
February 10 2011
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A correction has been published:
Understanding the yeast host cell response to recombinant membrane protein production
Citation
Zharain Bawa, Charlotte E. Bland, Nicklas Bonander, Nagamani Bora, Stephanie P. Cartwright, Michelle Clare, Matthew T. Conner, Richard A.J. Darby, Marvin V. Dilworth, William J. Holmes, Mohammed Jamshad, Sarah J. Routledge, Stephane R. Gross, Roslyn M. Bill; Understanding the yeast host cell response to recombinant membrane protein production. Biochem Soc Trans 1 June 2011; 39 (3): 719–723. doi: https://doi.org/10.1042/BST0390719
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