Sequence-specific DNA modification is of significance for applications in bio- and nano-technology, medical diagnostics and fundamental life sciences research. Preferentially, labelling should be performed covalently, which avoids doubts about label dissociation from the DNA under various conditions. Several methods to label native DNA have been developed in the last two decades. Triple-helix-forming oligodeoxynucleotides and hairpin polyamides that bind DNA sequences specifically in the major and minor groove respectively were used as targeting devices for subsequent covalent labelling. In addition, enzyme-directed labelling approaches utilizing nicking endonucleases in combination with DNA polymerases or DNA methyltransferases have been employed. This review summarizes various techniques useful for functionalization of long native DNA.
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April 2011
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Conference Article|
March 22 2011
Sequence-specific covalent labelling of DNA
Anna Gottfried;
Anna Gottfried
1Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany
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Elmar Weinhold
Elmar Weinhold
1
1Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52056 Aachen, Germany
1To whom correspondence should be addressed (email elmar.weinhold@oc.rwth-aachen.de).
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Publisher: Portland Press Ltd
Received:
January 04 2011
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (2): 623–628.
Article history
Received:
January 04 2011
Citation
Anna Gottfried, Elmar Weinhold; Sequence-specific covalent labelling of DNA. Biochem Soc Trans 1 April 2011; 39 (2): 623–628. doi: https://doi.org/10.1042/BST0390623
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