Tetraspanins are thought to exert their biological function(s) by co-ordinating the lateral movement and trafficking of associated molecules into tetraspanin-enriched microdomains. A second four-TM (transmembrane) domain protein family, the Claudin superfamily, is the major structural component of cellular TJs (tight junctions). Although the Claudin family displays low sequence homology and appears to be evolutionarily distinct from the tetraspanins, CD81 and Claudin-1 are critical molecules defining HCV (hepatitis C virus) entry; we recently demonstrated that CD81–Claudin-1 complexes have an essential role in this process. To understand the molecular basis of CD81–Claudin-1 complex formation, we produced and purified milligram quantities of full-length CD81 and Claudin-1, alone and in complex, in both detergent and lipid contexts. Structural characterization of these purified proteins will allow us to define the mechanism(s) underlying virus–cell interactions and aid the design of therapeutic agents targeting early steps in the viral life cycle.
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April 2011
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Conference Article|
March 22 2011
Structural characterization of CD81–Claudin-1 hepatitis C virus receptor complexes
Nicklas Bonander;
Nicklas Bonander
1
*School of Life and Health Sciences, Aston University, Birmingham B4 7ET, U.K.
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Mohammed Jamshad;
Mohammed Jamshad
1
*School of Life and Health Sciences, Aston University, Birmingham B4 7ET, U.K.
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Ke Hu;
Ke Hu
†College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, U.K.
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Michelle J. Farquhar;
Michelle J. Farquhar
†College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, U.K.
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Zania Stamataki;
Zania Stamataki
†College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, U.K.
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Peter Balfe;
Peter Balfe
†College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, U.K.
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Jane A. McKeating;
Jane A. McKeating
†College of Medical and Dental Science, University of Birmingham, Birmingham B15 2TT, U.K.
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Roslyn M. Bill
Roslyn M. Bill
2
*School of Life and Health Sciences, Aston University, Birmingham B4 7ET, U.K.
2To whom correspondence should be addressed (email r.m.bill@aston.ac.uk).
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Publisher: Portland Press Ltd
Received:
September 03 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (2): 537–540.
Article history
Received:
September 03 2010
Citation
Nicklas Bonander, Mohammed Jamshad, Ke Hu, Michelle J. Farquhar, Zania Stamataki, Peter Balfe, Jane A. McKeating, Roslyn M. Bill; Structural characterization of CD81–Claudin-1 hepatitis C virus receptor complexes. Biochem Soc Trans 1 April 2011; 39 (2): 537–540. doi: https://doi.org/10.1042/BST0390537
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