CRISPR (cluster of regularly interspaced palindromic repeats)/Cas and CRISPR/Cmr systems of Sulfolobus, targeting DNA and RNA respectively of invading viruses or plasmids are complex and diverse. We address their classification and functional diversity, and the wide sequence diversity of RAMP (repeat-associated mysterious protein)-motif containing proteins encoded in Cmr modules. Factors influencing maintenance of partially impaired CRISPR-based systems are discussed. The capacity for whole CRISPR transcripts to be generated despite the uptake of transcription signals within spacer sequences is considered. Targeting of protospacer regions of invading elements by Cas protein–crRNA (CRISPR RNA) complexes exhibit relatively low sequence stringency, but the integrity of protospacer-associated motifs appears to be important. Different mechanisms for circumventing or inactivating the immune systems are presented.
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February 2011
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Conference Article|
January 19 2011
CRISPR-based immune systems of the Sulfolobales: complexity and diversity
Roger A. Garrett;
Roger A. Garrett
1
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
1To whom correspondence should be addressed (email garrett@bio.ku.dk).
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Shiraz A. Shah;
Shiraz A. Shah
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Gisle Vestergaard;
Gisle Vestergaard
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Ling Deng;
Ling Deng
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Soley Gudbergsdottir;
Soley Gudbergsdottir
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Chandra S. Kenchappa;
Chandra S. Kenchappa
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Susanne Erdmann;
Susanne Erdmann
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Qunxin She
Qunxin She
1Archaea Centre, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200N Copenhagen K, Denmark
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Publisher: Portland Press Ltd
Received:
September 30 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (1): 51–57.
Article history
Received:
September 30 2010
Citation
Roger A. Garrett, Shiraz A. Shah, Gisle Vestergaard, Ling Deng, Soley Gudbergsdottir, Chandra S. Kenchappa, Susanne Erdmann, Qunxin She; CRISPR-based immune systems of the Sulfolobales: complexity and diversity. Biochem Soc Trans 1 February 2011; 39 (1): 51–57. doi: https://doi.org/10.1042/BST0390051
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