We have obtained draft genomic sequences of PD (partial denitrificant) and CD (complete denitrificant) strains of Thermus thermophilus. Their genomes are similar in size to that of the aerobic strains sequenced to date and probably contain a similar megaplasmid. In the CD strain, the genes encoding a putative cytochrome cd1 Nir (nitrite reductase) and ancillary proteins were clustered with a cytochrome c-dependent Nor (nitric oxide reductase), and with genes that are probably implicated in their regulation. The Nar (nitrate reductase) and associated genes were also clustered and located 7 kb downstream of the genes coding for the Nir. The whole nar–nir–nor denitrification supercluster was identified as part of a variable region of a megaplasmid. No homologues of NosZ were found despite nitrogen balance supports the idea that such activity actually exists.
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February 2011
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Conference Article|
January 19 2011
Partial and complete denitrification in Thermus thermophilus: lessons from genome drafts
Carlos Bricio;
Carlos Bricio
*Centro de Biología Molecular Severo Ochoa (UAM-CSIC), Universidad Autónoma de Madrid, 28049 Madrid, Spain
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Laura Alvarez;
Laura Alvarez
*Centro de Biología Molecular Severo Ochoa (UAM-CSIC), Universidad Autónoma de Madrid, 28049 Madrid, Spain
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Manuel J. Gómez;
Manuel J. Gómez
†Centro de Astrobiología (CSIC/INTA), Instituto Nacional de Técnica Aeroespacial, 28850 Torrejón de Ardoz, Madrid, Spain
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José Berenguer
José Berenguer
1
*Centro de Biología Molecular Severo Ochoa (UAM-CSIC), Universidad Autónoma de Madrid, 28049 Madrid, Spain
1To whom correspondence should be addressed (email jberenguer@cbm.uam.es).
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Publisher: Portland Press Ltd
Received:
September 09 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem Soc Trans (2011) 39 (1): 249–253.
Article history
Received:
September 09 2010
Citation
Carlos Bricio, Laura Alvarez, Manuel J. Gómez, José Berenguer; Partial and complete denitrification in Thermus thermophilus: lessons from genome drafts. Biochem Soc Trans 1 February 2011; 39 (1): 249–253. doi: https://doi.org/10.1042/BST0390249
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