One of the principal problems facing nucleic acid delivery systems using polyplexes is the instability of the complexes in the presence of proteins and high salt concentrations. We have used a cross-linking polymer to overcome this problem. Pendant thiol moieties have been incorporated into a PAA (polyamidoamine) homopolymer and a PEG [poly(ethylene glycol)]–PAA–PEG copolymer reported previously as a self-assembling system. When mixed with DNA, small monodisperse sterically stabilized particles are formed in quantitative yields. Optimization of the formulation resulted in nanoparticles which are stable in seawater. This cross-linked formulation has been successfully tested in both freshwater and estuarine field trials as a water tracer. Future work will develop these particles as a groundwater tracer and also for therapeutic applications of nucleic acid delivery.
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August 2009
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Conference Article|
July 22 2009
Sterically stabilized self-assembling reversibly cross-linked polyelectrolyte complexes with nucleic acids for environmental and medical applications
Martin C. Garnett;
Martin C. Garnett
1
*School of Pharmacy, University Park, University of Nottingham, Nottingham NG7 2RD, U.K.
1To whom correspondence should be addressed (email martin.garnett@nottingham.ac.uk).
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Paolo Ferruti;
Paolo Ferruti
†Department of Organic and Industrial Chemistry, University of Milan, Via Venezian 21, 20133 Milan, Italy
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Elisabetta Ranucci;
Elisabetta Ranucci
†Department of Organic and Industrial Chemistry, University of Milan, Via Venezian 21, 20133 Milan, Italy
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Marco A. Suardi;
Marco A. Suardi
†Department of Organic and Industrial Chemistry, University of Milan, Via Venezian 21, 20133 Milan, Italy
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Mieke Heyde;
Mieke Heyde
‡EnviroGene Limited, Tredomen Innovation & Technology Centre, Tredomen Business Park, Ystrad Mynach, Hengoed CF82 7FQ, Wales, U.K.
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Rob Sleat
Rob Sleat
‡EnviroGene Limited, Tredomen Innovation & Technology Centre, Tredomen Business Park, Ystrad Mynach, Hengoed CF82 7FQ, Wales, U.K.
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Publisher: Portland Press Ltd
Received:
January 05 2009
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (4): 713–716.
Article history
Received:
January 05 2009
Citation
Martin C. Garnett, Paolo Ferruti, Elisabetta Ranucci, Marco A. Suardi, Mieke Heyde, Rob Sleat; Sterically stabilized self-assembling reversibly cross-linked polyelectrolyte complexes with nucleic acids for environmental and medical applications. Biochem Soc Trans 1 August 2009; 37 (4): 713–716. doi: https://doi.org/10.1042/BST0370713
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