We have been studying the functions of PCNA (proliferating-cell nuclear antigen) for the assembly and reassembly of the replisome during replication fork progression. We have identified the functional interactions between PCNA and several proteins involved in DNA replication and repair from Pyrococcus furiosus. We recently reported that the activity of UDG (uracil–DNA glycosylase) in P. furiosus (PfuUDG) is stimulated by PCNA (PfuPCNA) in vitro, and identified an atypical PCNA-binding site, AKTLF, in the PfuUDG protein. To understand further the function of the complex in the BER (base excision repair) process, we investigated the AP (apurinic/apyrimidinic) endonuclease, which can process the BER pathway after uracil removal by UDG. Interestingly, one candidate ORF (open reading frame) for the AP endonuclease was found in the operon containing the gene encoding UDG in the P. furiosus genome. However, this ORF did not exhibit any activity. Instead, we identified the AP endonuclease activity from the other candidate gene products, and designated the protein as PfuAP. We discovered a physical interaction between PfuAP and PfuPCNA, suggesting the formation of a BER complex in one of the repair systems in P. furiosus.
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February 2009
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Conference Article|
January 20 2009
Studies on the base excision repair (BER) complex in Pyrococcus furiosus
Shinichi Kiyonari;
Shinichi Kiyonari
*Department of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
†BIRD-Japan Science and Technology Agency, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
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Saki Tahara;
Saki Tahara
*Department of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
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Maiko Uchimura;
Maiko Uchimura
*Department of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
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Tsuyoshi Shirai;
Tsuyoshi Shirai
‡Department of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama-shi, Shiga 526-0829, Japan
§BIRD-Japan Science and Technology Agency, 1266 Tamura-cho, Nagahama-shi, Shiga 526-0829, Japan
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Sonoko Ishino;
Sonoko Ishino
*Department of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
†BIRD-Japan Science and Technology Agency, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
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Yoshizumi Ishino
*Department of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
†BIRD-Japan Science and Technology Agency, 6-10-1 Hakozaki, Fukuoka-shi, Fukuoka 812-8581, Japan
2To whom correspondence should be addressed (email ishino@agr.kyushu-u.ac.jp).
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Publisher: Portland Press Ltd
Received:
October 03 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 79–82.
Article history
Received:
October 03 2008
Citation
Shinichi Kiyonari, Saki Tahara, Maiko Uchimura, Tsuyoshi Shirai, Sonoko Ishino, Yoshizumi Ishino; Studies on the base excision repair (BER) complex in Pyrococcus furiosus. Biochem Soc Trans 1 February 2009; 37 (1): 79–82. doi: https://doi.org/10.1042/BST0370079
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