MBF1 (multiprotein bridging factor 1) is a highly conserved protein in archaea and eukaryotes. It was originally identified as a mediator of the eukaryotic transcription regulator BmFTZ-F1 (Bombyx mori regulator of fushi tarazu). MBF1 was demonstrated to enhance transcription by forming a bridge between distinct regulatory DNA-binding proteins and the TATA-box-binding protein. MBF1 consists of two parts: a C-terminal part that contains a highly conserved helix–turn–helix, and an N-terminal part that shows a clear divergence: in eukaryotes, it is a weakly conserved flexible domain, whereas, in archaea, it is a conserved zinc-ribbon domain. Although its function in archaea remains elusive, its function as a transcriptional co-activator has been deduced from thorough studies of several eukaryotic proteins, often indicating a role in stress response. In addition, MBF1 was found to influence translation fidelity in yeast. Genome context analysis of mbf1 in archaea revealed conserved clustering in the crenarchaeal branch together with genes generally involved in gene expression. It points to a role of MBF1 in transcription and/or translation. Experimental data are required to allow comparison of the archaeal MBF1 with its eukaryotic counterpart.
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February 2009
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Conference Article|
January 20 2009
Role of multiprotein bridging factor 1 in archaea: bridging the domains?
Bart de Koning;
Bart de Koning
1
1Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
1To whom correspondence should be addressed (email Bart.deKoning@wur.nl).
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Fabian Blombach;
Fabian Blombach
2Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
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Hao Wu;
Hao Wu
2
3Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
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Stan J.J. Brouns;
Stan J.J. Brouns
4Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
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John van der Oost
John van der Oost
5Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
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Publisher: Portland Press Ltd
Received:
August 04 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 52–57.
Article history
Received:
August 04 2008
Citation
Bart de Koning, Fabian Blombach, Hao Wu, Stan J.J. Brouns, John van der Oost; Role of multiprotein bridging factor 1 in archaea: bridging the domains?. Biochem Soc Trans 1 February 2009; 37 (1): 52–57. doi: https://doi.org/10.1042/BST0370052
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