The two archaea Ignicoccus hospitalis and Nanoarchaeum equitans form a unique intimate association, the character of which is not yet fully understood. Electron microscopic investigations show that at least two modes of cell–cell interactions exist: (i) the two cells are interconnected via thin fibres; and (ii) the two cell surfaces are in direct contact with each other. In order to shed further light on the molecules involved, we isolated a protein complex, by using detergent-induced solubilization of cell envelopes, followed by a combination of chromatography steps. Analysis by MS and comparison with databases revealed that this fraction contained two dominant proteins, representing the respective major envelope proteins of the two archaea. In addition, a considerable set of membrane proteins is specifically associated with these proteins. They are now the focus of further biochemical and ultrastructural investigations.
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February 2009
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Conference Article|
January 20 2009
The interaction of Nanoarchaeum equitans with Ignicoccus hospitalis: proteins in the contact site between two cells
Tillmann Burghardt;
Tillmann Burghardt
*Centre of Electron Microscopy, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany
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Benjamin Junglas;
Benjamin Junglas
*Centre of Electron Microscopy, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany
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Frank Siedler;
Frank Siedler
†Membrane Biochemistry, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany
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Reinhard Wirth;
Reinhard Wirth
‡Lehrstuhl für Mikrobiologie, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany
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Harald Huber;
Harald Huber
‡Lehrstuhl für Mikrobiologie, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany
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Reinhard Rachel
Reinhard Rachel
1
*Centre of Electron Microscopy, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany
1To whom correspondence should be addressed (email reinhard.rachel@biologie.uni-regensburg.de).
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Publisher: Portland Press Ltd
Received:
November 20 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 127–132.
Article history
Received:
November 20 2008
Citation
Tillmann Burghardt, Benjamin Junglas, Frank Siedler, Reinhard Wirth, Harald Huber, Reinhard Rachel; The interaction of Nanoarchaeum equitans with Ignicoccus hospitalis: proteins in the contact site between two cells. Biochem Soc Trans 1 February 2009; 37 (1): 127–132. doi: https://doi.org/10.1042/BST0370127
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