There are a large number of proteins involved in the control of eukaryotic DNA replication, which act together to ensure DNA is replicated only once every cell cycle. Key proteins involved in the initiation and elongation phases of DNA replication include the MCM (minchromosome maintenance) proteins, MCM2–MCM7, a family of six related proteins believed to act as the replicative helicase. Genome sequencing has revealed that the archaea possess a simplified set of eukaryotic replication homologues. The complexity of the DNA replication machinery in eukaryotes has led to a number of archaeal species being adapted as model organisms for the study of the DNA replication process. Most archaea sequenced to date possess a single MCM homologue that forms a hexameric complex. Recombinant MCMs from several archaea have been used in the biochemical characterization of the protein, revealing that the MCM complex has ATPase, DNA-binding and -unwinding activities. Unusually, the genome of the methanogenic archaeon Methanococcus maripaludis contains four MCM homologues, all of which contain the conserved motifs required for function. The availability of a wide range of genetic tools for the manipulation of M. maripaludis and the relative ease of growth of this organism in the laboratory makes it a good potential model for studying the role of multiple MCMs in DNA replication.
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February 2009
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Conference Article|
January 20 2009
Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?
Alison D. Walters;
Alison D. Walters
1Department of Biology (Area 5), PO Box 373, University of York, York YO10 5YW, U.K.
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James P.J. Chong
James P.J. Chong
1
1Department of Biology (Area 5), PO Box 373, University of York, York YO10 5YW, U.K.
1To whom correspondence should be addressed (email jpjc1@york.ac.uk).
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Publisher: Portland Press Ltd
Received:
August 04 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 1–6.
Article history
Received:
August 04 2008
Citation
Alison D. Walters, James P.J. Chong; Methanococcus maripaludis: an archaeon with multiple functional MCM proteins?. Biochem Soc Trans 1 February 2009; 37 (1): 1–6. doi: https://doi.org/10.1042/BST0370001
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