There is a growing appreciation for the diverse roles of zinc as a signalling substance in biological systems. Zinc signalling is brought about by changes in intracellular concentrations of labile Zn2+, resulting in both genomic and non-genomic effects. The genomic responses are largely mediated by MTF1 (metal-regulatory transcription factor 1), which binds to MREs (metal-response elements) in the 5′ regulatory region of genes in response to zinc. Treatment of cultured zebrafish ZF4 cells with siRNA (small interfering RNA) to MTF1 changed the transcriptional response to zinc for over 1000 genes, as assessed using an oligonucleotide microarray. From this primary list of MTF1-dependent genes, we identified a relatively small cohort that showed a configuration of MREs in their 5′ regulatory regions similar to known MTF1 targets. This group showed a remarkable dominance of nucleic acid-binding proteins and other proteins involved in embryological development, implicating MTF1 as a master regulator of gene expression during development.
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December 2008
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Conference Article|
November 19 2008
Zinc-controlled gene expression by metal-regulatory transcription factor 1 (MTF1) in a model vertebrate, the zebrafish
Christer Hogstrand;
Christer Hogstrand
1
*Nutritional Sciences Division, School of Biomedical and Health Sciences, King's College London, Franklin–Wilkins Building, 150 Stamford Street, London SE1 9NH, U.K.
1To whom correspondence should be addressed (email christer.hogstrand@kcl.ac.uk).
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Dongling Zheng;
Dongling Zheng
*Nutritional Sciences Division, School of Biomedical and Health Sciences, King's College London, Franklin–Wilkins Building, 150 Stamford Street, London SE1 9NH, U.K.
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Graham Feeney;
Graham Feeney
†Cardiff School of Biosciences, Cardiff University, P.O. Box 911, Cardiff CF10 3US, U.K.
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Phil Cunningham;
Phil Cunningham
*Nutritional Sciences Division, School of Biomedical and Health Sciences, King's College London, Franklin–Wilkins Building, 150 Stamford Street, London SE1 9NH, U.K.
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Peter Kille
Peter Kille
†Cardiff School of Biosciences, Cardiff University, P.O. Box 911, Cardiff CF10 3US, U.K.
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Publisher: Portland Press Ltd
Received:
June 11 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem Soc Trans (2008) 36 (6): 1252–1257.
Article history
Received:
June 11 2008
Citation
Christer Hogstrand, Dongling Zheng, Graham Feeney, Phil Cunningham, Peter Kille; Zinc-controlled gene expression by metal-regulatory transcription factor 1 (MTF1) in a model vertebrate, the zebrafish. Biochem Soc Trans 1 December 2008; 36 (6): 1252–1257. doi: https://doi.org/10.1042/BST0361252
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