β-Arrestins 1 and 2 are ubiquitously expressed intracellular adaptor and scaffolding proteins that play important roles in GPCR (G-protein-coupled receptor) desensitization, internalization, intracellular trafficking and G-protein-independent signalling. Recent developments in BRET (bioluminescence resonance energy transfer) technology enable novel insights to be gained from real-time monitoring of GPCR–β-arrestin complexes in live cells for prolonged periods. In concert with confocal microscopy, assays for studying internalization and recycling kinetics such as ELISAs, and techniques for measuring downstream signalling pathways such as those involving MAPKs (mitogen-activated protein kinases), investigators can now use a range of experimental tools to elucidate the ever-expanding roles of β-arrestins in mediating GPCR function.
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August 2007
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Conference Article|
July 20 2007
Monitoring interactions between G-protein-coupled receptors and β-arrestins
K.D.G. Pfleger;
K.D.G. Pfleger
1
*7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia
1To whom correspondence should be addressed (email kpfleger@waimr.uwa.edu.au).
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M.B. Dalrymple;
M.B. Dalrymple
*7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia
†Keogh Institute for Medical Research, QEII Medical Centre, Nedlands, Perth, WA 6009, Australia
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J.R. Dromey;
J.R. Dromey
*7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia
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K.A. Eidne
K.A. Eidne
*7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia
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Publisher: Portland Press Ltd
Received:
April 23 2007
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem Soc Trans (2007) 35 (4): 764–766.
Article history
Received:
April 23 2007
Citation
K.D.G. Pfleger, M.B. Dalrymple, J.R. Dromey, K.A. Eidne; Monitoring interactions between G-protein-coupled receptors and β-arrestins. Biochem Soc Trans 1 August 2007; 35 (4): 764–766. doi: https://doi.org/10.1042/BST0350764
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