CYP106A2 from Bacillus megaterium ATCC 13368 is a bacterial cytochrome P450 that is capable of transforming steroid hormones. It can be easily expressed in Escherichia coli with a high yield. Its activity in vitro can be achieved by using the adrenal redox proteins adrenodoxin and adrenodoxin reductase. So far, it was not possible to crystallize CYP106A2 because of degradation during the crystallization process. Nevertheless, CYP106A2 is an interesting enzyme for biotechnological use. It hydroxylates pharmaceutically important steroids such as progesterone and 11-deoxycortisol. However, it will be necessary for efficient application of CYP106A2 in biotechnology to improve the hydroxylation activity and manipulate the regiospecificity. The present paper gives an overview of recent developments in protein engineering of CYP106A2.
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December 2006
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Conference Article|
October 25 2006
Function and engineering of the 15β-hydroxylase CYP106A2
C. Virus;
C. Virus
1Naturwissenschaftlich-Technische Fakultät III, Institut für Biochemie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany
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M. Lisurek;
M. Lisurek
1Naturwissenschaftlich-Technische Fakultät III, Institut für Biochemie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany
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B. Simgen;
B. Simgen
1Naturwissenschaftlich-Technische Fakultät III, Institut für Biochemie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany
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F. Hannemann;
F. Hannemann
1Naturwissenschaftlich-Technische Fakultät III, Institut für Biochemie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany
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R. Bernhardt
R. Bernhardt
1
1Naturwissenschaftlich-Technische Fakultät III, Institut für Biochemie, Universität des Saarlandes, Postfach 151150, 66041 Saarbrücken, Germany
1To whom correspondence should be addressed (email ritabern@mx.uni-saarland.de).
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Publisher: Portland Press Ltd
Received:
June 30 2006
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (6): 1215–1218.
Article history
Received:
June 30 2006
Citation
C. Virus, M. Lisurek, B. Simgen, F. Hannemann, R. Bernhardt; Function and engineering of the 15β-hydroxylase CYP106A2. Biochem Soc Trans 1 December 2006; 34 (6): 1215–1218. doi: https://doi.org/10.1042/BST0341215
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