Cytochrome P450 mono-oxygenases play prominent roles in a diverse set of metabolic pathways, but the function of most of these enzymes remains obscure. A bottleneck in the functional genomics of this superfamily constitutes hypothesis generation to identify potential substrates (or substrate classes) individual P450s may act on. We used publicly available large-scale expression data to perform co-expression analysis comparing the expression matrix of each P450 with those from more than 4000 selected genes across thousands of microarrays. Based on functional annotations of co-expressed genes from a diverse set of databases, co-expressed pathways were thus identified for each P450. Using this approach, most P450s with known functions were placed into their respective pathways, thereby proofing the concept. As examples, pathway mapping results identifying novel P450s potentially acting on flower-specific monoterpenes and root-specific triterpenes are described. Co-expression results for all Arabidopsis P450s will be presented as a web resource on the ‘CYPedia’ web pages (http://ibmp.u-strasbg.fr/~CYPedia/).
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December 2006
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Conference Article|
October 25 2006
Functional annotation of the Arabidopsis P450 superfamily based on large-scale co-expression analysis
J. Ehlting;
J. Ehlting
1
*IBMP (Institut de Biologie Moléculaire des Plantes), CNRS (Centre National de la Recherche Scientifique), 28 rue Goethe, 67000 Strasbourg, France
1To whom correspondence should be addressed (email juergen.ehlting@ibmp-ulp.u-strasbg.fr).
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N.J. Provart;
N.J. Provart
†Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada M5S 3B2
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D. Werck-Reichhart
D. Werck-Reichhart
*IBMP (Institut de Biologie Moléculaire des Plantes), CNRS (Centre National de la Recherche Scientifique), 28 rue Goethe, 67000 Strasbourg, France
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Publisher: Portland Press Ltd
Received:
July 20 2006
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (6): 1192–1198.
Article history
Received:
July 20 2006
Citation
J. Ehlting, N.J. Provart, D. Werck-Reichhart; Functional annotation of the Arabidopsis P450 superfamily based on large-scale co-expression analysis. Biochem Soc Trans 1 December 2006; 34 (6): 1192–1198. doi: https://doi.org/10.1042/BST0341192
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