Sushi peptides [S1 (Sushi 1 peptide) and S3] are derived from the LPS (lipopolysaccharide; also known as endotoxin)-binding domains of an LPS-sensitive serine protease, Factor C, from the horseshoe crab (Carcinoscorpius rotundicauda). S1 and S3 interact at high affinity with LPS. The intermolecular disulphide bonding in the S3 dimer is indispensable for its LPS binding, disruption and consequent neutralization. Simultaneously, the specific interaction between the Sushi peptides and bacterial membrane phospholipids further explains the selective propensity of these peptides for the Gram-negative bacteria. Our findings yield insights into a complex molecular paradigm in which the juxtaposition of LPS molecules and the anionic phospholipid POPG (1-palmitoyl-2-oleoyl phosphatidylglycerol) on the bacterial outer membrane confers such interfacial properties which create the optimal environment for the interaction between the peptides and bacterial membrane lipids.
Skip Nav Destination
Article navigation
April 2006
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
March 20 2006
The specificity of Sushi peptides for endotoxin and anionic phospholipids: potential application of POPG as an adjuvant for anti-LPS strategies
P. Li;
P. Li
*Department of Biological Sciences, National University of Singapore, Singapore 117543
Search for other works by this author on:
M. Sun;
M. Sun
*Department of Biological Sciences, National University of Singapore, Singapore 117543
Search for other works by this author on:
B. Ho;
B. Ho
1
†Department of Microbiology, National University of Singapore, Singapore 117543
Search for other works by this author on:
J.L. Ding
*Department of Biological Sciences, National University of Singapore, Singapore 117543
2To whom correspondence should be addressed (email dbsdjl@nus.edu.sg).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 02 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (2): 270–272.
Article history
Received:
November 02 2005
Citation
P. Li, M. Sun, B. Ho, J.L. Ding; The specificity of Sushi peptides for endotoxin and anionic phospholipids: potential application of POPG as an adjuvant for anti-LPS strategies. Biochem Soc Trans 1 April 2006; 34 (2): 270–272. doi: https://doi.org/10.1042/BST0340270
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.