The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron microscopy at the squid giant synapse. Procedures that prevent clathrin from assembling into membrane coats, such as impairment of binding of the AP180 and AP-2 adaptor proteins, completely prevent membrane budding during endocytosis. These procedures also reduce exocytosis, presumably an indirect effect of a reduction in the number of SVs following block of endocytosis. Disrupting the binding of auxilin to Hsc70 (heat-shock cognate 70) prevents clathrin-coated vesicles from uncoating and also disrupts SV recycling. Taken together, these results indicate that a clathrin-dependent pathway is the primary means of SV recycling at this synapse under physiological conditions.
Skip Nav Destination
Article navigation
February 2006
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
January 20 2006
Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse
G.J. Augustine;
G.J. Augustine
1
* Department of Neurobiology, Duke University School of Medicine, Box 3209, Durham, NC 27710, U.S.A.
† Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
1 To whom correspondence should be addressed (email georgea@neuro.duke.edu).
Search for other works by this author on:
J.R. Morgan;
J.R. Morgan
† Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
‡ Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, U.S.A.
Search for other works by this author on:
C.A. Villalba-Galea;
C.A. Villalba-Galea
* Department of Neurobiology, Duke University School of Medicine, Box 3209, Durham, NC 27710, U.S.A.
† Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
Search for other works by this author on:
S. Jin;
S. Jin
§ Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, U.S.A.
Search for other works by this author on:
K. Prasad;
K. Prasad
† Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
§ Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, U.S.A.
Search for other works by this author on:
E.M. Lafer
E.M. Lafer
† Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
§ Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, U.S.A.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 22 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (1): 68–72.
Article history
Received:
September 22 2005
Citation
G.J. Augustine, J.R. Morgan, C.A. Villalba-Galea, S. Jin, K. Prasad, E.M. Lafer; Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse. Biochem Soc Trans 1 February 2006; 34 (1): 68–72. doi: https://doi.org/10.1042/BST0340068
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.