The AF0174–AF0176 gene cluster in Archaeoglobus fulgidus encodes a putative oxyanion reductase of the D-type (Type II) family of molybdo-enzymes. Sequence analysis reveals that the catalytic subunit AF0176 shares low identity (31–32%) and similarity (41–42%) to both NarG and SerA, the catalytic components of the respiratory nitrate and selenate reductases respectively. Consequently, predicting the oxyanion substrate selectivity of AF0176 has proved difficult based solely on sequence alignments. In the present study, we have modelled both AF0176 and SerA on the recently determined X-ray structure of the NAR (nitrate reductase) from Escherichia coli and have identified a number of key amino acid residues, conserved in all known NAR sequences, including AF0176, that we speculate may enhance selectivity towards trigonal planar (NO3−) rather than tetrahedral (SeO42− and ClO4−) substrates.
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February 2006
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Conference Article|
January 20 2006
Developing structure-based models to predict substrate specificity of D-group (Type II) molybdenum enzymes: application to a molybdo-enzyme of unknown function from Archaeoglobus fulgidus
E.J. Dridge;
E.J. Dridge
* Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, U.K.
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D.J. Richardson;
D.J. Richardson
† School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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R.J. Lewis;
R.J. Lewis
* Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, U.K.
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C.S. Butler
C.S. Butler
1
* Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, U.K.
1 To whom correspondence should be addressed (email c.s.butler@ncl.ac.uk).
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Publisher: Portland Press Ltd
Received:
September 22 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2006 The Biochemical Society
2006
Biochem Soc Trans (2006) 34 (1): 118–121.
Article history
Received:
September 22 2005
Citation
E.J. Dridge, D.J. Richardson, R.J. Lewis, C.S. Butler; Developing structure-based models to predict substrate specificity of D-group (Type II) molybdenum enzymes: application to a molybdo-enzyme of unknown function from Archaeoglobus fulgidus. Biochem Soc Trans 1 February 2006; 34 (1): 118–121. doi: https://doi.org/10.1042/BST0340118
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