Pantothenate (vitamin B5) is a water-soluble vitamin essential for the synthesis of CoA and ACP (acyl-carrier protein, cofactors in energy yielding reactions including carbohydrate metabolism and fatty acid synthesis. Pantothenate is synthesized de novo by plants and micro-organisms; however, animals obtain the vitamin through their diet. Utilizing our knowledge of the pathway in Escherichia coli, we have discovered and cloned genes encoding the first and last enzymes of the pathway from Arabidopsis, panB1, panB2 and panC. It is unlikely that there is a homologue of the E. coli panD gene, therefore plants must make β-alanine by an alternative route. Possible candidates for the remaining gene, panE, are being investigated. GFP (green fluorescent protein) fusions of the three identified plant enzymes have been generated and the subcellular localization of the enzymes studied. Work is now being performed to elucidate expression patterns of the transcripts and characterize the proteins encoded by these genes.
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August 2005
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Conference Article|
August 01 2005
Pantothenate biosynthesis in higher plants
K.M. Coxon;
K.M. Coxon
*Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, U.K.
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E. Chakauya;
E. Chakauya
*Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, U.K.
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H.H. Ottenhof;
H.H. Ottenhof
*Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, U.K.
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H.M. Whitney;
H.M. Whitney
*Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, U.K.
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T.L. Blundell;
T.L. Blundell
†Department of Biochemistry, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.
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C. Abell;
C. Abell
‡University Chemical Laboratory, Lensfield Road, Cambridge CB2 1EW, U.K.
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A.G. Smith
A.G. Smith
1
*Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, U.K.
1To whom correspondence should be addressed (email as25@cam.ac.uk).
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Publisher: Portland Press Ltd
Received:
April 28 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (4): 743–746.
Article history
Received:
April 28 2005
Citation
K.M. Coxon, E. Chakauya, H.H. Ottenhof, H.M. Whitney, T.L. Blundell, C. Abell, A.G. Smith; Pantothenate biosynthesis in higher plants. Biochem Soc Trans 1 August 2005; 33 (4): 743–746. doi: https://doi.org/10.1042/BST0330743
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