O-glycosylation is thought to play a significant role in the regulation of cell growth. However, only limited information is available, and few specific and selective inhibitors have been found. We have synthesized a library of O-glycosylation inhibitors based on benzyl-O-N-acetyl-D-galactosamine. These inhibitors were tested with an established series of human colorectal cancer cell lines, which model the adenoma-carcinoma sequence. Cancer cells were incubated with the inhibitors, and examined for cell growth patterns, and cellular and subcellular glycosylation using a range of lectins with confocal microscopy. The specificity of O-glycan inhibition was confirmed for the library, relative to other forms of glycosylation. All inhibitors tested resulted in smaller cell yields. However, a differential effect on O-glycosylation was detected using the lectins showing variation of localization at a subcellular level in the various cell lines. Further differential action of the inhibitor library was observed for apoptosis and on the cell cycle with the cell lines tested. This work demonstrates that O-glycosylation is closely involved in the regulation of cell growth in colorectal cancer cells and that the generation of a library of low-molecular-mass inhibitors offers a valuable means of examining this regulation at the molecular level.
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August 2005
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Conference Article|
August 01 2005
Action of a library of O-glycosylation inhibitors on the growth of human colorectal cancer cells in culture
G. Patsos;
G. Patsos
*Mucin Research Group, Clinical Science, South Bristol, Bristol BS2 8HW, U.K.
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V. Hebbe-Viton;
V. Hebbe-Viton
†Department of Chemistry, University of Bristol, Bristol BS8 1TS, U.K.
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R. San Martin;
R. San Martin
†Department of Chemistry, University of Bristol, Bristol BS8 1TS, U.K.
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C. Paraskeva;
C. Paraskeva
‡Department of Pathology and Microbiology, University of Bristol, Bristol BS8 1TD, U.K.
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T. Gallagher;
T. Gallagher
†Department of Chemistry, University of Bristol, Bristol BS8 1TS, U.K.
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A. Corfield
A. Corfield
1
*Mucin Research Group, Clinical Science, South Bristol, Bristol BS2 8HW, U.K.
1To whom correspondence should be addressed (email tony.corfield@bristol.ac.uk).
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Publisher: Portland Press Ltd
Received:
March 22 2005
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (4): 721–723.
Article history
Received:
March 22 2005
Citation
G. Patsos, V. Hebbe-Viton, R. San Martin, C. Paraskeva, T. Gallagher, A. Corfield; Action of a library of O-glycosylation inhibitors on the growth of human colorectal cancer cells in culture. Biochem Soc Trans 1 August 2005; 33 (4): 721–723. doi: https://doi.org/10.1042/BST0330721
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