In recent years, it has become evident that [Fe-S] proteins, such as hydrogenase, nitrogenase and aconitase, require a complex machinery to assemble and insert their associated [Fe-S] clusters. So far, three different types of [Fe-S] cluster biosynthetic systems have been identified and these have been designated nif, isc and suf. In the present work, we show that the nif-specific [Fe-S] cluster biosynthetic system from Azotobacter vinelandii, which is required for nitrogenase maturation, cannot functionally replace the isc [Fe-S] cluster system used for the maturation of other [Fe-S] proteins, such as aconitase. The results indicate that, in certain cases, [Fe-S] cluster biosynthetic machineries have evolved to perform only specialized functions.
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Conference Article|
February 01 2005
NifU and NifS are required for the maturation of nitrogenase and cannot replace the function of isc-gene products in Azotobacter vinelandii
D.C. Johnson;
D.C. Johnson
1Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061-0346, U.S.A.
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P.C. Dos Santos;
P.C. Dos Santos
1Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061-0346, U.S.A.
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D.R. Dean
D.R. Dean
1
1Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061-0346, U.S.A.
1To whom correspondence should be addressed (email deandr@vt.edu).
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Publisher: Portland Press Ltd
Received:
October 04 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 90–93.
Article history
Received:
October 04 2004
Citation
D.C. Johnson, P.C. Dos Santos, D.R. Dean; NifU and NifS are required for the maturation of nitrogenase and cannot replace the function of isc-gene products in Azotobacter vinelandii. Biochem Soc Trans 1 February 2005; 33 (1): 90–93. doi: https://doi.org/10.1042/BST0330090
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