The genome of the yeast Saccharomyces cerevisiae encodes the essential protein Nar1p that is conserved in virtually all eukaryotes and exhibits striking sequence similarity to bacterial iron-only hydrogenases. Previously, we have shown that Nar1p is an Fe-S protein and that assembly of its co-factors depends on the mitochondrial Fe-S cluster biosynthesis apparatus. Using functional studies in vivo, we demonstrated that Nar1p has an essential role in the maturation of cytosolic and nuclear, but not of mitochondrial, Fe-S proteins [Balk, Pierik, Aguilar Netz, Mühlenhoff and Lill (2004) EMBO J. 23, 2105–2115]. Here we provide further spectroscopic evidence that Nar1p possesses two Fe-S clusters. We also show that Nar1p is required for Fe-S cluster assembly on the P-loop NTPase Nbp35p, another newly identified component of the cytosolic Fe-S protein assembly machinery. These data suggest a complex biochemical pathway of extra-mitochondrial Fe-S protein biogenesis involving unique eukaryotic proteins.
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Conference Article|
February 01 2005
Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis
J. Balk;
J. Balk
*Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033 Marburg, Germany
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A.J. Pierik;
A.J. Pierik
†Laboratorium für Mikrobiologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse 8, D-35043 Marburg, Germany
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D.J. Aguilar Netz;
D.J. Aguilar Netz
*Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033 Marburg, Germany
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U. Mühlenhoff;
U. Mühlenhoff
*Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033 Marburg, Germany
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R. Lill
R. Lill
1
*Institut für Zytobiologie und Zytopathologie, Philipps-Universität Marburg, Robert-Koch-Strasse 6, D-35033 Marburg, Germany
1To whom correspondence should be addressed (email Lill@mailer.uni-marburg.de).
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Publisher: Portland Press Ltd
Received:
September 30 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 86–89.
Article history
Received:
September 30 2004
Citation
J. Balk, A.J. Pierik, D.J. Aguilar Netz, U. Mühlenhoff, R. Lill; Nar1p, a conserved eukaryotic protein with similarity to Fe-only hydrogenases, functions in cytosolic iron-sulphur protein biogenesis. Biochem Soc Trans 1 February 2005; 33 (1): 86–89. doi: https://doi.org/10.1042/BST0330086
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