In this work, we describe the hexokinase 2 (Hxk2) signalling pathway within the yeast cell. Hxk2 and Mig1 are the two major factors of glucose repression in Saccharomyces cerevisiae. The functions of both proteins have been extensively studied but there is no information about possible interactions among them in the repression pathway. Our results demonstrate that Hxk2 interacts directly with Mig1 in vivo and in vitro and that the ten amino acids motif between K6 and M15 is required for their interaction. This interaction has been detected at the DNA level both in vivo by chromatin immunoprecipitation experiments and in vitro using purified proteins and a DNA fragment containing the MIG1 site of the SUC2 promoter. This demonstrates that the interaction is of physiological relevance. Our findings show that the main role of Hxk2 in the glucose signalling pathway is the interaction with Mig1 to generate a repressor complex located in the nucleus of S. cerevisiae.
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February 2005
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Conference Article|
February 01 2005
Glucose sensing through the Hxk2-dependent signalling pathway
F. Moreno;
F. Moreno
1
1Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
1To whom correspondence should be addressed (email fmoreno@uniovi.es).
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D. Ahuatzi;
D. Ahuatzi
1Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
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A. Riera;
A. Riera
1Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
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C.A. Palomino;
C.A. Palomino
1Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
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P. Herrero
P. Herrero
1Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
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Publisher: Portland Press Ltd
Received:
September 17 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 265–268.
Article history
Received:
September 17 2004
Citation
F. Moreno, D. Ahuatzi, A. Riera, C.A. Palomino, P. Herrero; Glucose sensing through the Hxk2-dependent signalling pathway. Biochem Soc Trans 1 February 2005; 33 (1): 265–268. doi: https://doi.org/10.1042/BST0330265
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