Nitric oxide reduction in Ralstonia eutropha H16 is catalysed by the quinol-dependent NO reductase NorB. norB and the adjacent norA form an operon that is controlled by the σ54-dependent transcriptional activator NorR in response to NO. A NorR derivative containing MalE in place of the N-terminal domain binds to a 73 bp region upstream of norA that includes three copies of the putative upstream activator sequence GGT-(N7)-ACC. Mutations altering individual bases of this sequence resulted in an 80–90% decrease in transcriptional activation by wild-type NorR. Similar motifs are present in several proteobacteria upstream of genes encoding proteins of NO metabolism. The N-terminal domain of NorR contains a GAF module and is hypothesized to interact with a signal molecule. A NorR derivative lacking this domain activates the norAB promoter constitutively. Amino acid exchanges within the GAF module identified a cysteine residue that is essential for promoter activation by NorR. Signal sensing by NorR is negatively modulated by the iron-containing protein NorA.
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Conference Article|
February 01 2005
Transcriptional regulation of nitric oxide reduction in Ralstonia eutropha H16
A. Büsch;
A. Büsch
1Institut für Biologie/Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, 10115 Berlin, Germany
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K. Strube;
K. Strube
1Institut für Biologie/Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, 10115 Berlin, Germany
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B. Friedrich;
B. Friedrich
1Institut für Biologie/Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, 10115 Berlin, Germany
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R. Cramm
R. Cramm
1
1Institut für Biologie/Mikrobiologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, 10115 Berlin, Germany
1To whom correspondence should be addressed (email rainer.cramm@rz.hu-berlin.de).
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Publisher: Portland Press Ltd
Received:
September 02 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 193–194.
Article history
Received:
September 02 2004
Citation
A. Büsch, K. Strube, B. Friedrich, R. Cramm; Transcriptional regulation of nitric oxide reduction in Ralstonia eutropha H16. Biochem Soc Trans 1 February 2005; 33 (1): 193–194. doi: https://doi.org/10.1042/BST0330193
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