In denitrifying bacteria, the concentration of NO is maintained low by a tight control of the expression and activity of nitrite and NO reductases. Regulation involves redox-linked transcription factors, such as those belonging to the CRP-FNR (cAMP receptor protein–fumarate and nitrate reductase regulator) superfamily, which act as oxygen and N-oxide sensors. Given that few members of this superfamily have been characterized in detail, we have cloned, expressed and purified the dissimilative nitrate respiration regulator from Pseudomonas aeruginosa. To gain insights on the structural properties of the dissimilative nitrate respiration regulator, we have also determined the aggregation state of the purified protein and its ability to bind hydrophobic compounds such as 8-anilino-1-naphthalenesulphonic acid.
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February 2005
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Conference Article|
February 01 2005
N-oxide sensing in Pseudomonas aeruginosa: expression and preliminary characterization of DNR, an FNR–CRP type transcriptional regulator
S. Rinaldo;
S. Rinaldo
1Department of Biochemical Sciences ‘A. Rossi Fanelli’, University of Rome La Sapienza, P.le A. Moro 5, 00185 Rome, Italy
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G. Giardina;
G. Giardina
1Department of Biochemical Sciences ‘A. Rossi Fanelli’, University of Rome La Sapienza, P.le A. Moro 5, 00185 Rome, Italy
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M. Brunori;
M. Brunori
1Department of Biochemical Sciences ‘A. Rossi Fanelli’, University of Rome La Sapienza, P.le A. Moro 5, 00185 Rome, Italy
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F. Cutruzzolà
F. Cutruzzolà
1
1Department of Biochemical Sciences ‘A. Rossi Fanelli’, University of Rome La Sapienza, P.le A. Moro 5, 00185 Rome, Italy
1To whom correspondence should be addressed (email francesca.cutruzzola@uniroma1.it).
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Publisher: Portland Press Ltd
Received:
September 05 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 184–186.
Article history
Received:
September 05 2004
Citation
S. Rinaldo, G. Giardina, M. Brunori, F. Cutruzzolà; N-oxide sensing in Pseudomonas aeruginosa: expression and preliminary characterization of DNR, an FNR–CRP type transcriptional regulator. Biochem Soc Trans 1 February 2005; 33 (1): 184–186. doi: https://doi.org/10.1042/BST0330184
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