Rhizobium etli CFN42 is not capable of growing anaerobically with nitrate but it grows with nitrite as a terminal electron acceptor. This bacterium contains the nirK gene encoding the copper-containing Nir (nitrite reductase), which is located on the cryptic plasmid pCFN42f. Mutational analysis has demonstrated that a nirK deficient mutant was not capable of growing under nitrite-respiring conditions. Moreover, microaerobic growth of this mutant was inhibited by the presence of nitrite. Nir activity and nitrite uptake were highly diminished in a nirK mutant, compared with the wild-type levels after incubation under anaerobic conditions. Our results suggest that the copper-containing Nir may have both a respiratory and a nitrite-detoxifying role in R. etli.
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Conference Article|
February 01 2005
Function of the Rhizobium etli CFN42 nirK gene in nitrite metabolism
E. Bueno;
E. Bueno
*Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080 Granada, Spain
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N. Gómez-Hernández;
N. Gómez-Hernández
†Centro de Investigación sobre Fijación de Nitrógeno, UNAM, P.O. Box 565-A, Cuernavaca, Morelos 62271, México
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L. Girard;
L. Girard
†Centro de Investigación sobre Fijación de Nitrógeno, UNAM, P.O. Box 565-A, Cuernavaca, Morelos 62271, México
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E.J. Bedmar;
E.J. Bedmar
*Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080 Granada, Spain
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M.J. Delgado
M.J. Delgado
1
*Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, P.O. Box 419, 18080 Granada, Spain
1To whom correspondence should be addressed (email mdelgado@eez.csic.es).
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Publisher: Portland Press Ltd
Received:
September 24 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 162–163.
Article history
Received:
September 24 2004
Citation
E. Bueno, N. Gómez-Hernández, L. Girard, E.J. Bedmar, M.J. Delgado; Function of the Rhizobium etli CFN42 nirK gene in nitrite metabolism. Biochem Soc Trans 1 February 2005; 33 (1): 162–163. doi: https://doi.org/10.1042/BST0330162
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