The napEDABC genes of Bradyrhizobium japonicum encode the periplasmic nitrate reductase, an Mo-containing enzyme which catalyses the reduction of nitrate to nitrite when oxygen concentrations are limiting. In this bacterium, another set of genes, modABC, code for a high affinity ABC-type Mo transport system. A B. japonicum modA mutant has been obtained that is not capable of growing anaerobically with nitrate and lacks nitrate reductase activity. Under nitrate respiring conditions, when Mo concentrations are limiting, the B. japonicum modA mutant lacked both the 90 kDa protein corresponding to the NapA component of the periplasmic nitrate reductase, and the membrane-bound 25 kDa c-type cytochrome NapC. Regulatory studies using a napE–lacZ fusion indicated that napE expression was highly reduced in the modA mutant background when the cells were incubated anaerobically with nitrate under Mo-deficient conditions.
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Conference Article|
February 01 2005
Molybdate-dependent expression of the periplasmic nitrate reductase in Bradyrhizobium japonicum
N. Bonnard;
N. Bonnard
*Fachbereich Biologie der Philipps Universität Marburg, Zellbiologie und Angewandte Botanik, Karl-von-Frisch-Str. 8, 35032 Marburg, Germany
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A. Tresierra-Ayala;
A. Tresierra-Ayala
†Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080-Granada, Spain
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E.J. Bedmar;
E.J. Bedmar
†Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080-Granada, Spain
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M.J. Delgado
M.J. Delgado
1
†Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, E-18080-Granada, Spain
1To whom correspondence should be addressed (email mdelgado@eez.csic.es).
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Publisher: Portland Press Ltd
Received:
September 24 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 127–129.
Article history
Received:
September 24 2004
Citation
N. Bonnard, A. Tresierra-Ayala, E.J. Bedmar, M.J. Delgado; Molybdate-dependent expression of the periplasmic nitrate reductase in Bradyrhizobium japonicum. Biochem Soc Trans 1 February 2005; 33 (1): 127–129. doi: https://doi.org/10.1042/BST0330127
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