The eukaryotic green alga, Chlamydomonas reinhardtii, produces H2 under anaerobic conditions, in a reaction catalysed by an [FeFe]-hydrogenase. To identify genes that influence H2 production in C. reinhardtii, a library of 6000 colonies on agar plates was screened with sensitive chemochromic H2-sensor films for clones defective in H2 production. Two mutants of particular interest were fully characterized. One mutant, hydEF-1, is unable to assemble an active [FeFe]-hydrogenase. This is the first reported C. reinhardtii mutant that is not capable of producing any H2. The second mutant, sta7-10, is not able to accumulate insoluble starch and has significantly lowered H2-photoproduction rates in comparison with the wild-type. In hydEF-1, anaerobiosis induces transcription of the two reported C. reinhardtii hydrogenase genes, HydA1 and HydA2, indicating a normal transcriptional response to anaerobiosis. In contrast, the transcription of both hydrogenase genes in sta7-10 is significantly attenuated.
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Conference Article|
February 01 2005
Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii
M.C. Posewitz;
M.C. Posewitz
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
†Department of Environmental Science and Engineering, Colorado School of Mines, Golden, CO 80401, U.S.A.
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P.W. King;
P.W. King
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
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S.L. Smolinski;
S.L. Smolinski
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
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R. Davis Smith, II;
R. Davis Smith, II
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
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A.R. Ginley;
A.R. Ginley
1
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
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M.L. Ghirardi;
M.L. Ghirardi
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
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M. Seibert
M. Seibert
2
*National Renewable Energy Laboratory, 1617 Cole Blvd, Golden, CO 80401, U.S.A.
2To whom correspondence should be addressed (email Mike_seibert@nrel.gov).
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Publisher: Portland Press Ltd
Received:
September 30 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2005 The Biochemical Society
2005
Biochem Soc Trans (2005) 33 (1): 102–104.
Article history
Received:
September 30 2004
Citation
M.C. Posewitz, P.W. King, S.L. Smolinski, R. Davis Smith, A.R. Ginley, M.L. Ghirardi, M. Seibert; Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii. Biochem Soc Trans 1 February 2005; 33 (1): 102–104. doi: https://doi.org/10.1042/BST0330102
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