Complexes of α2A-ARs (α2A-adrenergic receptors) and MORs (μ-opioid receptors), probably hetero-oligomers, were detected by co-immunoisolation after extraction from HEK-293 cells (human embryonic kidney 293 cells). Functional communication between these receptors is revealed by α2A-AR activation of a pertussis toxin-insensitive Giα subunit (termed as Gi1) when fused with the MOR and evaluated in membranes from pertussis toxin-treated cells. However, the α2A-AR does not require transactivation through MOR, since quantitatively indistinguishable results were observed in cells co-expressing α2A-AR and a fusion protein of Gi1 with the first transmembrane span of MOR (myc–MOR-TM1). Functional cross-talk among these α2A-AR–MOR complexes does not occur for internalization profiles; incubation with adrenaline (epinephrine) leads to endocytosis of α2A-AR but not MOR, while incubation with DAMGO ([D-Ala,NMe-Phe,Gly-ol]enkephalin) leads to endocytosis of MOR but not α2A-AR in cells co-expressing both the receptors. Hence, α2A-AR and MOR hetero-oligomers, although they occur, do not have an obligatory functional influence on one another in the paradigms studied.
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Conference Article|
October 26 2004
Hetero-oligomers of α2A-adrenergic and μ-opioid receptors do not lead to transactivation of G-proteins or altered endocytosis profiles
Y.Q. Zhang;
Y.Q. Zhang
1Department of Pharmacology and Center for Molecular Neuroscience, Vanderbilt University Medical Center, 2200 Pierce Ave., 464A RRB, Nashville, TN 37232-6600, U.S.A.
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L.E. Limbird
L.E. Limbird
1
1Department of Pharmacology and Center for Molecular Neuroscience, Vanderbilt University Medical Center, 2200 Pierce Ave., 464A RRB, Nashville, TN 37232-6600, U.S.A.
1To whom correspondence should addressed (email lee.limbird@vanderbilt.edu).
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Publisher: Portland Press Ltd
Received:
June 22 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (5): 856–860.
Article history
Received:
June 22 2004
Citation
Y.Q. Zhang, L.E. Limbird; Hetero-oligomers of α2A-adrenergic and μ-opioid receptors do not lead to transactivation of G-proteins or altered endocytosis profiles. Biochem Soc Trans 1 November 2004; 32 (5): 856–860. doi: https://doi.org/10.1042/BST0320856
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