β-N-Acetylhexosaminidase from a filamentous fungus Aspergillus oryzae is a secreted enzyme known to be an important component of the binary chitinolytic system. Cloning of the hexA gene and sequencing of the enzyme revealed its unique preproprotein structure. While the enzyme's zincin-like and catalytic domain had significant similarities with members of the glycohydrolase 20 family, the propeptide was unique for the fungal enzyme. Detailed pulse–chase and inhibition studies revealed that propeptide was processed during the biosynthesis of the enzyme. Moreover, the presence of propeptide was necessary for enzyme activation, dimerization and secretion. The catalytic unit was N-glycosylated, and the propeptide was O-glycosylated, both in their C-terminal parts. Deglycosylation experiments revealed that the N-glycosylation increased the stability and solubility of the enzyme. In contrast, O-glycosylated propeptide was necessary to attain the full enzymic activity.
Skip Nav Destination
Article navigation
November 2004
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
October 26 2004
N-glycosylated catalytic unit meets O-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase
O. Plíhal;
O. Plíhal
1
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
1To whom correspondence should be addressed (email plihal@biomed.cas.cz).
Search for other works by this author on:
J. Sklenář;
J. Sklenář
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
Search for other works by this author on:
J. Kmoníčková;
J. Kmoníčková
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
Search for other works by this author on:
P. Man;
P. Man
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
Search for other works by this author on:
P. Pompach;
P. Pompach
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
Search for other works by this author on:
V. Havlíček;
V. Havlíček
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
Search for other works by this author on:
V. Křen;
V. Křen
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
Search for other works by this author on:
K. Bezouška
K. Bezouška
*Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220 Praha 4, Czech Republic
†Department of Biochemistry, Faculty of Sciences, Charles University Prague, Hlavova 8, CZ-12840 Praha 2, Czech Republic
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 08 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (5): 764–765.
Article history
Received:
July 08 2004
Citation
O. Plíhal, J. Sklenář, J. Kmoníčková, P. Man, P. Pompach, V. Havlíček, V. Křen, K. Bezouška; N-glycosylated catalytic unit meets O-glycosylated propeptide: complex protein architecture in a fungal hexosaminidase. Biochem Soc Trans 1 November 2004; 32 (5): 764–765. doi: https://doi.org/10.1042/BST0320764
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.