In polycistronic genes, uORFs (upstream open reading frames) within the 5′-transcript leader sequence of major ORFs may regulate the translation of these major ORFs. In this case, ribosome reinitiates translation at a start codon of downstream ORF after translation termination of uORF. The plant RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit. In the RPL24-deficient mutant of Arabidopsis, short valve (stv), the basal region of the ovary is shortened, whereas the gynophore appears elongated. This phenotype is never seen in known mutants of other ribosomal protein genes, suggesting that RPL24 has a specific role in gynoecium development. Similar phenotypes were observed in the ett (ettin) and mp (monopteros) mutants. Both ETT and MP genes possess uORFs. We examined the hypothesis that these uORFs regulate their downstream major ORFs by a transient expression assay in Arabidopsis mesophyll protoplasts. Our results supported the idea, suggesting that the structural defects of the gynoecium in stv mutants were caused by decreased efficiency of translation reinitiation of ETT and/or MP.
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Conference Article|
August 01 2004
A key factor of translation reinitiation, ribosomal protein L24, is involved in gynoecium development in Arabidopsis
T. Nishimura;
T. Nishimura
*Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
†Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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T. Wada;
T. Wada
†Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
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K. Okada
K. Okada
1
*Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
†Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
‡Core Research for Evolutional Science and Technology (CREST) Research Project, Japan Science and Technology Agency, Kawaguchi, Saitama 322-0012, Japan
1To whom correspondence should be addressed (email kiyo@ok-lab.bot.kyoto-u.ac.jp).
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Publisher: Portland Press Ltd
Received:
April 27 2004
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 The Biochemical Society
2004
Biochem Soc Trans (2004) 32 (4): 611–613.
Article history
Received:
April 27 2004
Citation
T. Nishimura, T. Wada, K. Okada; A key factor of translation reinitiation, ribosomal protein L24, is involved in gynoecium development in Arabidopsis. Biochem Soc Trans 1 August 2004; 32 (4): 611–613. doi: https://doi.org/10.1042/BST0320611
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