CD43 down-regulation during the apoptosis of PMN (polymorphonuclear cells) is not caused by proteolysis or internalization. Could it be released with bleb-derived membrane vesicles? Membrane blebbing was followed by microscopy on PMN ‘synchronized’ by an overnight incubation at 15°C before their spontaneous apoptosis at 37°C. Released vesicles were quantified by flow cytometry. Membrane blebbing, release of bleb-derived membrane vesicles, decrease of CD43/CD16 expression and phosphatidylserine externalization occurred simultaneously. However, caspase and PKC inhibition prevented annexin binding but not blebbing, vesicle release or CD43 expression decrease; myosin light chain kinase inhibition prevented cell blebbing and vesicle release but had no effect on CD43/CD16 down-regulation or annexin V binding. By electron microscopy, CD43 appeared poorly expressed on membrane blebs and concentrated at bleb ‘necks’. In conclusion, CD43 down-regulation is not caused by cell blebbing. Cell blebbing, phospholipid ‘flip-flop’ and CD43/CD16 down-regulation are independent membrane events.
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Conference Article|
June 01 2004
Early membrane events in polymorphonuclear cell (PMN) apoptosis: membrane blebbing and vesicle release, CD43 and CD16 down-regulation and phosphatidylserine externalization
P. Nusbaum;
P. Nusbaum
*INSERM U 507 Hôpital Necker, Paris, France
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C. Lainé;
C. Lainé
*INSERM U 507 Hôpital Necker, Paris, France
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P. Lesavre;
P. Lesavre
*INSERM U 507 Hôpital Necker, Paris, France
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L. Halbwachs-Mecarelli
L. Halbwachs-Mecarelli
1
*INSERM U 507 Hôpital Necker, Paris, France
1To whom correspondence should be addressed (email mecarelli@necker.fr).
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 Biochemical Society
2004
Biochem Soc Trans (2004) 32 (3): 477–479.
Citation
P. Nusbaum, C. Lainé, S. Seveau, P. Lesavre, L. Halbwachs-Mecarelli; Early membrane events in polymorphonuclear cell (PMN) apoptosis: membrane blebbing and vesicle release, CD43 and CD16 down-regulation and phosphatidylserine externalization. Biochem Soc Trans 1 June 2004; 32 (3): 477–479. doi: https://doi.org/10.1042/bst0320477
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