Endothelial-cell dysfunction is a critical initiating event in the pathogenesis of atherosclerosis. Although there is evidence to suggest that chylomicron remnants (CMRs), lipoproteins derived from the diet, influence endothelial-cell function to generate a pro-atherogenic phenotype, the mechanisms involved remain undefined. We have examined the effects of CMR-like particles (CMR-LPs) on human endothelial-cell function, focusing on the cyclo-oxygenase (COX) and nitric oxide synthase (NOS) pathways. CMR-LPs strongly enhanced the expression of the inducible cyclo-oxygenase COX-2 and increased prostacyclin synthesis in a biphasic manner. Studies with the COX-2-selective inhibitor NS-398 confirmed the COX-2 dependency of the later increase in prostanoid production. Pre-incubation with CMR-LPs reduced basal and thrombin-stimulated cGMP generation, whereas expression of endothelial NOS was not modified by remnant treatment.
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Conference Article|
February 01 2004
Chylomicron-remnant-like particles modify production of vasoactive mediators by endothelial cells
M. Evans;
M. Evans
1
Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.
1To whom correspondence should be addressed (e-mail mevans@rvc.ac.uk).
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Y. Berhane;
Y. Berhane
Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.
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K.M. Botham;
K.M. Botham
Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.
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J. Elliott;
J. Elliott
Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.
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C.P.D. Wheeler-Jones
C.P.D. Wheeler-Jones
Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2004 Biochemical Society
2004
Biochem Soc Trans (2004) 32 (1): 110–112.
Citation
M. Evans, Y. Berhane, K.M. Botham, J. Elliott, C.P.D. Wheeler-Jones; Chylomicron-remnant-like particles modify production of vasoactive mediators by endothelial cells. Biochem Soc Trans 1 February 2004; 32 (1): 110–112. doi: https://doi.org/10.1042/bst0320110
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