The monitoring of the movements of membrane proteins (or lipids) by single-particle tracking enables one to obtain reliable insights into the complex dynamic organization of the plasma membrane constituents. Using this technique, we investigated the diffusional behaviour of a G-protein-coupled receptor. The trajectories of the receptors revealed a diffusion mode combining a short-term rapid confined diffusion with a long-term slow diffusion. A detailed statistical analysis shows that the receptors have a diffusion confined to a domain which itself diffuses, the confinement being due to long-range attractive inter-protein interactions. The existing models of the dynamic organization of the cell membrane cannot explain our results. We propose a theoretical Brownian model of interacting proteins that is consistent with the experimental observations and accounts for the variations found as a function of the domain size of the short-term and long-term diffusion coefficients.
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October 2003
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Conference Article|
October 01 2003
Interprotein interactions are responsible for the confined diffusion of a G-protein-coupled receptor at the cell surface
F. Daumas;
F. Daumas
*Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205, route de Narbonne, 31077 Toulouse cedex, France
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N. Destainville;
N. Destainville
†Laboratoire de Physique Théorique, IRSAMC, CNRS FRE 2603, 118, route de Narbonne, 31062 Toulouse cedex, France
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C. Millot;
C. Millot
*Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205, route de Narbonne, 31077 Toulouse cedex, France
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A. Lopez;
A. Lopez
*Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205, route de Narbonne, 31077 Toulouse cedex, France
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D. Dean;
D. Dean
†Laboratoire de Physique Théorique, IRSAMC, CNRS FRE 2603, 118, route de Narbonne, 31062 Toulouse cedex, France
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L. Salomé
L. Salomé
1
*Institut de Pharmacologie et Biologie Structurale, UMR CNRS 5089, 205, route de Narbonne, 31077 Toulouse cedex, France
1To whom correspondence should be addressed (e-mail Laurence.Salome@ipbs.fr).
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2003 Biochemical Society
2003
Biochem Soc Trans (2003) 31 (5): 1001–1005.
Citation
F. Daumas, N. Destainville, C. Millot, A. Lopez, D. Dean, L. Salomé; Interprotein interactions are responsible for the confined diffusion of a G-protein-coupled receptor at the cell surface. Biochem Soc Trans 1 October 2003; 31 (5): 1001–1005. doi: https://doi.org/10.1042/bst0311001
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