We have compared calcium mobilization by Ins(1,4,5)P3(IP3), cADP-ribose (cADPR) and nicotinic acid–adenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca2+ uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that all calcium messengers induce Ca2+ release from the nuclear envelope. Pre-treatment of nuclei with thapsigargin completely abolished the responses to the calcium messengers, indicating that Ca2+ stores in isolated nuclei are thapsigargin-sensitive. Using different pharmacological tools, we show that Ca2+ release from pancreatic nuclei is unlikely to occur from stores other than those with endoplasmic reticulum characteristics. We conclude that all three calcium messengers can release Ca2+ from pancreatic acinar nuclear stores, as previously shown for IP3 and cADPR. It would appear that NAADP releases Ca2+ from the same IP3- and cADPR-sensitive stores with endoplasmic reticulum characteristics.
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February 2003
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Conference Article|
February 01 2003
Calcium signalling in and around the nuclear envelope
J. Gerasimenko;
J. Gerasimenko
1
*Medical Research Council Secretory Control Research Group, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, U.K.
1Correspondence may be addressed to either author (e-mail o.v.gerasimenko@liv.ac.uk or j.v.gerasimenko@liv.ac.uk).
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Y. Maruyama;
Y. Maruyama
†Department of Physiology, Tohoku University School of Medicine, Sendai 980–8575, Japan
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A. Tepikin;
A. Tepikin
*Medical Research Council Secretory Control Research Group, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, U.K.
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O.H. Petersen;
O.H. Petersen
*Medical Research Council Secretory Control Research Group, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, U.K.
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O. Gerasimenko
O. Gerasimenko
1
*Medical Research Council Secretory Control Research Group, Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, U.K.
1Correspondence may be addressed to either author (e-mail o.v.gerasimenko@liv.ac.uk or j.v.gerasimenko@liv.ac.uk).
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
Copyright 2003 Biochemical Society
2003
Biochem Soc Trans (2003) 31 (1): 76–78.
Citation
J. Gerasimenko, Y. Maruyama, A. Tepikin, O.H. Petersen, O. Gerasimenko; Calcium signalling in and around the nuclear envelope. Biochem Soc Trans 1 February 2003; 31 (1): 76–78. doi: https://doi.org/10.1042/bst0310076
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