The Nrampl (natural resistance-associated macrophage protein 1) gene modulates the growth of intracellular pathogens and encodes a divalent cation transporter within lysosomes/late endosomes of macrophages. Nrampl modulates the cytoplasmic iron pool. Wu, Polack and Dalla-Favera [(1999) Science 283, 676–679] showed reciprocal control of H-ferritin and IRP2 by c-Myc, and suggest that c-Myc regulates genes to increase cytoplasmic iron. A role for c-Myc in Nrampl regulation was evaluated. Co-transfection studies show that c-Myc represses Nrampl promoter function. Five non-canonical Myc-max binding sites (E-box) identified within the Nrampl 5′-flanking sequence are not responsible for the inhibitory effects of c-Myc on Nrampl expression. An initiator(s) adjacent to the transcriptioninitiation site is a candidate for the inhibition observed. Results are consistent with a role for Nrampl removing iron from the cytosol and antagonizing c-Myc function.
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August 2002
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Conference Article|
August 01 2002
c-Myc represses the murine Nrampl promoter
H. Bowen;
H. Bowen
*Biochemistry and Molecular Biology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
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T. E. Biggs;
T. E. Biggs
*Biochemistry and Molecular Biology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
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S. T. Baker;
S. T. Baker
*Biochemistry and Molecular Biology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
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E. Phillips;
E. Phillips
*Biochemistry and Molecular Biology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
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V. H. Perry;
V. H. Perry
†Cell Sciences CNS Inflammation Group, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
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D. A. Mann;
D. A. Mann
‡Liver Group, Division of Cell and Molecular Medicine, Level D South Block, University of Southampton, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, U.K.
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C. H. Barton
C. H. Barton
1
*Biochemistry and Molecular Biology, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
1To whom correspondence should be addressed (e-mail CHB@soton.ac.uk)
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Publisher: Portland Press Ltd
Received:
March 06 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 774–777.
Article history
Received:
March 06 2002
Citation
H. Bowen, T. E. Biggs, S. T. Baker, E. Phillips, V. H. Perry, D. A. Mann, C. H. Barton; c-Myc represses the murine Nrampl promoter. Biochem Soc Trans 1 August 2002; 30 (4): 774–777. doi: https://doi.org/10.1042/bst0300774
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