The budding yeast Saccharomyces cerevisiae responds to growth in limiting amounts of iron by activating the transcription factor Aft1p and expressing a set of genes that ameliorate the effects of iron deprivation. Analysis of iron-regulated gene expression using cDNA microarrays has revealed the set of genes controlled by iron and Aft1p. Many of these genes are involved in the uptake of siderophore-bound iron from the environment. One family of genes, FIT1, FIT2 and FIT3, codes for mannoproteins that are incorporated into the cell wall via glycosylphosphatidylinositol anchors. These genes are involved in the retention of siderophore-iron in the cell wall. Siderophore-bound iron can be taken up into the cell via two genetically separable systems. One system requires the reduction and release of the iron from the siderophore prior to uptake by members of the Fre family of plasma-membrane metalloreductases. Following reduction and release from the siderophore, the iron is then taken up via the high-affinity ferrous transport system. A set of transporters that specifically recognizes siderophore-iron chelates is also expressed under conditions of iron deprivation. These transporters, encoded by ARN1, ARN2/TAF1, ARN3/SIT1 and ARN4/ENB1, facilitate the uptake of both hydroxamate- and catecholate-type siderophores. The Arn transporters are expressed in intracellular vesicles that correspond to the endosomal compartment, which suggests that intracellular trafficking of the siderophore and/or its transporter may be important for uptake.
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August 2002
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Conference Article|
August 01 2002
The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake
C. C. Philpott;
C. C. Philpott
1
1Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B-16, 10 Center Drive, Bethesda, MD 20892-1800, U.S.A.
1 To whom correspondence should be addressed (e-mail carolinep@intra.niddk.nih.gov)
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O. Protchenko;
O. Protchenko
1Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B-16, 10 Center Drive, Bethesda, MD 20892-1800, U.S.A.
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Y. W. Kim;
Y. W. Kim
1Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B-16, 10 Center Drive, Bethesda, MD 20892-1800, U.S.A.
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Y. Boretsky;
Y. Boretsky
1Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B-16, 10 Center Drive, Bethesda, MD 20892-1800, U.S.A.
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M. Shakoury-Elizeh
M. Shakoury-Elizeh
1Liver Diseases Section, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Building 10, Room 9B-16, 10 Center Drive, Bethesda, MD 20892-1800, U.S.A.
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Publisher: Portland Press Ltd
Received:
March 13 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 698–702.
Article history
Received:
March 13 2002
Citation
C. C. Philpott, O. Protchenko, Y. W. Kim, Y. Boretsky, M. Shakoury-Elizeh; The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake. Biochem Soc Trans 1 August 2002; 30 (4): 698–702. doi: https://doi.org/10.1042/bst0300698
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