Adenosylcobalamin or coenzyme B12-dependent enzymes are members of the still relatively small group of radical enzymes and catalyse 1,2-rearrangement reactions. A member of this family is methylmalonyl-CoA mutase, which catalyses the isomerization of methylmalonyl-CoA to succinyl-CoA and, unlike the others, is present in both bacteria and animals. Enzymes that catalyse some of the most chemically challenging reactions are the ones that tend to deploy radical chemistry. The use of radical intermediates in an active site lined with amino acid side chains that threaten to extinguish the reaction by presenting alternative groups for abstraction poses the conundrum of how the enzymes control their reactivity. In this review, insights into this issue that have emerged from kinetic, mutagenesis and structural studies are described for methylmalonyl-CoA mutase.
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August 2002
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Conference Article|
August 01 2002
Controlling the reactivity of radical intermediates by coenzyme B12-dependent methylmalonyl-CoA mutase
R. Banerjee;
R. Banerjee
1
1Biochemistry Department, University of Nebraska, Lincoln, NE 68588-0664, U.S.A.
1To whom correspondence should be addressed (e-mail rbanerjee|@unl.edu)
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M. Vlasie
M. Vlasie
1Biochemistry Department, University of Nebraska, Lincoln, NE 68588-0664, U.S.A.
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Publisher: Portland Press Ltd
Received:
April 10 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 621–624.
Article history
Received:
April 10 2002
Citation
R. Banerjee, M. Vlasie; Controlling the reactivity of radical intermediates by coenzyme B12-dependent methylmalonyl-CoA mutase. Biochem Soc Trans 1 August 2002; 30 (4): 621–624. doi: https://doi.org/10.1042/bst0300621
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