The calcitonin-gene-related peptide (CGRP) receptor component protein (RCP) is a 148-amino-acid intracellular protein that is required for G-protein-coupled signal transduction at receptors for the neuropeptide CGRP. RCP works in conjunction with two other proteins to constitute a functional CGRP receptor: calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1).CRLR has the stereotypical seven-transmembrane topology of a G-protein-coupled receptor; it requires RAMP1 for trafficking to the cell surface and for ligand specificity, and requires RCP for coupling to the cellular signal transduction pathway. We have made cell lines that expressed an antisense construct of RCP and determined that CGRP-mediated signal transduction was reduced, while CGRP binding was unaffected. Furthermore, signalling at two other endogenous G-protein-coupled receptors was unaffected, suggesting that RCP was specific for a limited subset of receptors.
Skip Nav Destination
Article navigation
August 2002
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
August 01 2002
Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction
M. A. Prado;
M. A. Prado
1Department of Physiology and Biophysics, University of Miami School of Medicine, P.O. Box 016430, Miami, FL 33101, U.S.A.
Search for other works by this author on:
B. Evans-Bain;
B. Evans-Bain
1Department of Physiology and Biophysics, University of Miami School of Medicine, P.O. Box 016430, Miami, FL 33101, U.S.A.
Search for other works by this author on:
I. M. Dickerson
I. M. Dickerson
1
1Department of Physiology and Biophysics, University of Miami School of Medicine, P.O. Box 016430, Miami, FL 33101, U.S.A.
1To whom correspondence should be addressed (e-mail imd@miami.edu)
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 11 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 460–464.
Article history
Received:
March 11 2002
Citation
M. A. Prado, B. Evans-Bain, I. M. Dickerson; Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction. Biochem Soc Trans 1 August 2002; 30 (4): 460–464. doi: https://doi.org/10.1042/bst0300460
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.