14-3-3 proteins belong to a family of conserved molecules, which play a regulatory role and participate in signal transduction and checkpoint control pathways. 14-3-3 proteins bind phosphoserine-phosphorylated ligands, such as the Raf-1 kinase and Bad, through recognition of the phosphorylated consensus motif, RSXpSXP (where pS is phosphoserine). Recently, a phosphorylation-independent interaction has been reported to occur between 14-3-3 and a small number of proteins, for example the 43 kDa inositol polyphosphate 5-phosphatase, glycoprotein Ib, p75NTR-associated cell-death executor (NADE) and the bacterial ADP-ribosyltransferase toxin exoenzyme S (ExoS). It has been suggested that specific residues of 14-3-3 proteins are required for activation of the bacterial toxin ExoS. An unphosphorylated peptide derived from a phage display library, known as the R18 peptide, and a synthetic peptide derived from ExoS inhibit the interaction between ExoS and 14-3-3. In this report we identify the amino acid sequence on ExoS which is responsible for its specific interaction with 14-3-3, both in vitro and in vivo. In addition, we believe that this interaction is critical for the ADP-ribosylation of an endogenous target, Ras, by ExoS both in vitro and in vivo. Loss of the 14-3-3-binding site on ExoS results in an ExoS molecule that is unable to efficiently inactivate Ras and shows a reduced capacity to change the morphology of infected cells, together with reduced killing activity.
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Conference Article|
August 01 2002
Exoenzyme S binds its cofactor 14-3-3 through a non-phosphorylated motif
B. Hallberg
B. Hallberg
1
1Department of Medical Biosciences/Pathology, Umeå University, S-901 87 Umeå, Sweden
1e-mail Bengt.Hallberg@cmb.umu.se
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Publisher: Portland Press Ltd
Received:
March 11 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 401–405.
Article history
Received:
March 11 2002
Citation
B. Hallberg; Exoenzyme S binds its cofactor 14-3-3 through a non-phosphorylated motif. Biochem Soc Trans 1 August 2002; 30 (4): 401–405. doi: https://doi.org/10.1042/bst0300401
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