The migration of vascular smooth muscle cells (VSMC) is known to be a key process in the development of a number of vascular lesions, although the precise mechanisms involved have still to be elucidated. In the present study, the production of endogenous fibronectins by VSMC migrating across intact and matrix-metalloproteinase-degraded collagen type I has been explored. Cellular fibronectin seems to play a role in the enhanced migration seen when VSMC are exposed to degraded collagen and platelet-derived growth factor-BB. VSMC were found to synthesize both exon IIIA-containing fibronectin (which predominated) and exon IIIB-containing fibronectin. When these cells were exposed to substrates consisting of recombinant exon IIIA-or exon IIIB-containing fibronectin, rates of migration were not elevated above those seen with undegraded collagen. Endogenous fibronectin production may thus be necessary, but not sufficient, for VSMC migration over degraded collagenous substrates.
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Conference Article|
April 01 2002
Role of newly synthesized fibronectin in vascular smooth muscle cell migration on matrix-metalloproteinase-degraded collagen
E. Stringa;
E. Stringa
*School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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D. White;
D. White
†Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, U.S.A.
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R. S. Tuan;
R. S. Tuan
†Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, U.S.A.
‡Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Disease, National Institutes of Health, Bethesda, MD 20892, U.S.A.
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V. Knauper;
V. Knauper
§Department of Biology, University of York, York YO10 5YW, U.K.
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J. Gavrilovic
J. Gavrilovic
1
*School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
1To whom correspondence should be addressed (e-mail J.Gavrilovic@uea.ac.uk).
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Publisher: Portland Press Ltd
Received:
December 17 2001
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (2): 102–107.
Article history
Received:
December 17 2001
Citation
E. Stringa, D. White, R. S. Tuan, V. Knauper, J. Gavrilovic; Role of newly synthesized fibronectin in vascular smooth muscle cell migration on matrix-metalloproteinase-degraded collagen. Biochem Soc Trans 1 April 2002; 30 (2): 102–107. doi: https://doi.org/10.1042/bst0300102
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